Postoperative pneumonia is one of the most common complications after lung cancer surgery and associated with a morbidity and mortality. Postoperative lymphopenia has been recently identified as one of risk factors for postoperative pneumonia. According to recent studies in polytrauma, cancer or septic shock, T cells dysfunction may be related to high expression of inhibitory receptors on lymphocytes.
This study will investigate prospectively TIM-3, PD-1 and CTLA4 expression on lymphocytes T cells before and after lung cancer surgery. Patients ≥ 18 years with no history of immunosuppressive state will be included.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
31
Blood samples will be collected in patients undergoing lung cancer surgery: * before lung cancer surgery, * the next day lung cancer surgery, * 3 days after lung cancer surgery. They will be treated in flow cytometry.
CHU Saint-Etienne
Saint-Etienne, France
PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes
Correlation between expression of PD-1, TIM-3, CTLA4 on CD4/CD8 lymphocytes and the occurrence of lymphopenia. Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry
Time frame: Days 0, 1, 3
functionality of CD4/CD8 lymphocytes and production of IF gamma, TNF and IL-2
Correlation between functionality of CD4/CD8 lymphocytes and production of interferon (IF) gamma, Tumor Necrosis Factor (TNF) and interleukine (IL-2). Measured by blood sample before lung cancer surgery, the next day lung cancer surgery and 3 days after lung cancer surgery. Analyzed by multicolour immunolabelling in flow cytometry
Time frame: Days 0, 1, 3
pneumonia
To analyse the occurrence of postoperative pneumonia.
Time frame: Days 3
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