In the present project the investigators will evaluate whether glucagonotropic properties of the gut-derived incretin hormone glucose-dependent insulinotropic polypeptide (GIP) may be utilized as a safeguard against hypoglycemia in patients with T1D.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
12
Steno Diabetes Center Copenhagen, Clinical Metabolic Physiology
Hellerup, Denmark
plasma glucose concentration
plasma glucose excursions and plasma glucose nadir
Time frame: At times (minute): - 30, - 15, 0, 15, 30, 45, 60, 75, 90, 105, 120, 135, 150, 165, 180, 195, 210, 225, 240 on study days
Glucose regulatory hormones
Counter regulatory hormones: glucagon, noradrenalin, cortisol, somatotropin, and insulin/c-peptide. Incremental and total area under the Concentration-Time Curve
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
Incretin hormones
Incretin hormones GLP-1 and GIP. Incremental and total area under the Concentration-Time Curve
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
GIP[3-30]
GIP\[3-30\]. Incremental and total area under the Concentration-Time Curve
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
Free fatty acids (FFA)
Free fatty acids(FFA). Incremental and total area under the Concentration-Time Curve
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
Blood analysis of paracetamol as an assessment of gastric emptying
Assessment of gastric emptying
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
Fat mRNA
Fat biopsy to evaluate changes in fat mRNA
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Time frame: At time 240 minute on study days
Fat Lipoprotein lipase (LPL)
Fat biopsy to evaluate LPL changes in fat
Time frame: At time 240 minute on study days
Fat Perilipin 4
Fat biopsy to evaluate Perilipin 4 changes in fat
Time frame: At time 240 minute on study days
Fat Fatty acid binding protein 4 (FABP4)
Fat biopsy to evaluate Fatty acid binding protein 4 (FABP4) changes in fat
Time frame: At time 240 minute on study days
Fat Hormonse-sensitive lipase (HSL)
Fat biopsy to evaluate Hormonse-sensitive lipase (HSL) changes in fat
Time frame: At time 240 minute on study days
Fat Vascular endothelial growth factor 4 (VEGF-A)
Fat biopsy to evaluate Vascular endothelial growth factor 4 (VEGF-A) changes in fat
Time frame: At time 240 minute on study days
Fat GIP receptor (GIPR)
Fat biopsy to evaluate GIP receptor (GIPR) changes in fat
Time frame: At time 240 minute on study days
Blood pressure
Changes in blood pressure, mm Hg
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days
Pulse
Changes in pulse, beats per minute
Time frame: At times (minute): - 30, 0, 15, 30, 60, 90, 120, 150, 180, 210, 240 on study days