Profibrosing Role of B Lymphocytes in Patients With Systemic Sclerosis.

University Hospital, Lille71 enrolled

Overview

B Lymphocytes are thought to play an important role in the pathophysiology of systemic sclerosis. In this study, the profibrosing role of B lymphocytes of patients with systemic sclerosis will be evaluated.

B Lymphocytes of patients with systemic sclerosis will be assessed in term of interaction with fibroblasts from patients with systemic sclerosis and compared to healthy controls.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Scleroderma, Systemic

Interventions

skin biopsyPROCEDURE

A skin biopsy will be performed in 10 patients in the form of 2 punches of 5 mm in the injured area in aseptic condition with a Rodnan score. In this study, 10 skin biopsies are needed, and will be performed on the first 10 patients accepting this biopsy.

Blood punctionOTHER

1 blood punction during the normal pathway of care of the patient. This punction will be used to perform the extraction of B lymphocyte

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: patients * systemic sclerosis according to ACR-EULAR 2013 * written consent * available insurance Healthy "blood" and healthy "biopsy" controls * written consent * available insurance Exclusion Criteria: patients * overlap with other connective tissue diseases * immunosuppressants in the past 12 months * corticosteroids ≥10mg/d * pregnancy or breast feeding Healthy "blood" and healthy "biopsy" controls * systemic sclerosis according to ACR-EULAR 2013 * another connective tissue diseases * immunosuppressants in the past 12 months * corticosteroids ≥10mg/d * pregnancy or breast feeding * With dysimmune inflammatory diseases (except thyroiditis) * Presenting an infection in progress or within the previous 15 days

Locations (1)

Hôpital Claude Huriez, CHU

Lille, France

Outcomes

Primary Outcomes

Fibroblast transcriptomic : myofibroblast signature

expression of alpha Smooth Muscle Actin

Time frame: 36 months

Secondary Outcomes

Fibroblast transcriptomic

expression of COL1A1, COL1A2, COL3A1 by quantitative RT-PCR

Time frame: 36 months

Difference in collagen infiltration by PicroSirius red staining between the skin biopsies of the two groups

Time frame: 36 months

Difference in the inflammatory infiltrate by immunohistological markings between the skin biopsies of the two groups

Time frame: 36 months

Data from ClinicalTrials.gov

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