The objective of this study was to establish a formulation, containing both Hibiscus sabdariffa L. (HS) and Lippia citriodora L. (LC) extracts (Metabolaid®) that had significant capacity to activate the AMPK-enzyme and to reduce triglyceride accumulation in the hypertrophied adipocyte model. Then, we assessed the efficacy of this combination in light-to-moderate-overweight subjects under risk of developing metabolic syndrome.
AMP-activated protein kinase (AMPK) has been postulated as a molecular target in the amelioration of obesity-related diseases, where most therapeutic approaches have failed. Plant-polyphenols have shown the capacity to ameliorate obesity-induced metabolic disturbances. A combination of polyphenols (LC-HS) derived from Hibiscus sabdariffa L. (HS) and Lippia citriodora L. (LC) (lemon verbena) (Metabolaid®) was assessed for triglyceride accumulation and AMPK activation in the hypertrophied adipocyte model 3T3-L1. A dietary supplement containing 500 mg of LC-HS was evaluated in a double blind, placebo-controlled and randomized trial in 56 overweight subjects for two months. Anthropometric and circulating biochemical parameters were determined.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
46
500 mg per day, in fasting conditions.
500 mg per day, in fasting conditions.
Universidad Miguel Hernandez de Elche
Elche, Alicante, Spain
Changes in body Weight, using a weight scale
In Kg
Time frame: At the beginning, four weeks and end of the intervention, total eight weeks.
Height, using measuring tape
In cm
Time frame: At the beginning, four weeks and eight weeks
BMI, calculated based on Weight and Height
in kg/m\^2
Time frame: At the beginning, four weeks and eight weeks
Waist Circumference, using a measuring tape
in kg/m\^2
Time frame: At the beginning, four weeks and eight weeks
Glucose
Blood Sampling in Fasting conditions, in mg/dl
Time frame: At the beginning, four weeks and eight weeks
Tryglicerides
Blood Sampling in Fasting conditions, in mg/dl
Time frame: At the beginning, four weeks and eight weeks
LDL Cholesterol
Blood Sampling in Fasting conditions, in mg/dl
Time frame: At the beginning, four weeks and eight weeks
HDL Cholesterol
Blood Sampling in Fasting conditions, in mg/dl
Time frame: At the beginning, four weeks and eight weeks
Total Cholesterol
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Blood Sampling in Fasting conditions, in mg/dl
Time frame: At the beginning, four weeks and eight weeks
Blood Pressure
Single Blood Pressure measurements, using the Omron M6 Comfort device
Time frame: At the beginning, four weeks and eight weeks
Heart rate
Single Blood Pressure measurements, using the Omron M6 Comfort device
Time frame: At the beginning, four weeks and eight weeks