Experiment 2: Understanding Liver Fat Metabolism

University of Oxford24 enrolled

Overview

There is a largely unsubstantiated thought in the literature that dietary unsaturated enter oxidation pathways preferentially compared to saturated fatty acids. This study is undertaking a direct comparison of the oxidation of dietary saturated and unsaturated fatty acids.

Provided subjects meet the inclusion criteria, then at least one week after the screening visit volunteers will then undertake the first two 'study days'. There will be two study days on separate occasions that will be up to 3 weeks apart. Each study day will be approximately 7 hours in duration. On each study day participants will be given a standardised 'test' meal that contains specially labelled fat molecules (stable-isotope tracer) and then repeated breath and blood samples will be taken over the course of the next 6 hours. The test meal consumed on each 'study day' will be identical in composition with only the specially labelled fat molecules being different (either unsaturated or saturated fat) and these will be given in a random order.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

DOUBLE

Enrollment

Conditions

Fatty Acid Oxidation

Interventions

Dietary fatty acid partitioningOTHER

There is no intervention as such, just a physiological assessment of the partitioning and oxidation of two different types of dietary fatty acids

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: Participant is willing and able to give informed consent for participation in the study. Male or Female, aged \>18 and \<65 years BMI \>19 \<35kg/m2 No medical condition or relevant drug therapy known to affect liver metabolism Exclusion Criteria: * • Age \<18 or \>65 years * Body mass index \<19 or \>35kg/m2 * A blood haemoglobin \<120mg/dL * Any metabolic condition or relevant drug therapy * Smoking * History of alcoholism or a greater than recommended alcohol intake * Pregnant or nursing mothers * Women prescribed hormone replacement therapy (HRT) or who have used this within the last 12 months * History of severe claustrophobia * Presence of metallic implants, pacemaker * Haemorrhagic disorders * Anticoagulant treatment

Locations (1)

Oxford Centre for Diabetes, Endocrinology and Metabolism

Oxford, United Kingdom

Outcomes

Primary Outcomes

Dietary fatty acid partitioning

The appearance of the 13Clabelled fatty acid in plasma triglycerides

Time frame: 6 hours

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.