Numerous studies found that anesthetic methods can influence the recurrence of tumor and the overall survival of patients after primary cancer surgery. Radiofrequency (RF) ablation is now widely used in clinic for treatment of hepatocellular carcinoma (HCC). Currently, diverse anesthetic methods, including general anesthesia (GA), epidural anesthesia and local anesthesia (LA), are used for RF ablation surgery. Using serum from HCC surgery patients randomized to receive either GA or LA during surgery, we investigated the effects of anesthetic methods on proliferation, migration and metastasis in HepG2 hepatic cancer cells in vitro.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
24
Patients will receive standard GA or LA for RF surgery.
Renji Hospital affliated to Shanghai Jiaotong University School of Medicine
Shanghai, Shanghai Municipality, China
cell proliferation
HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA for 24h and cell proliferation will be measured
Time frame: 1 hour post-surgery
cell migration
HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell migration will be measured
Time frame: 1 hour post-surgery
cell metastasis
HepG2 cells will be cultured with serum from patients undergoing RF ablation under GA or LA and cell metastasis will be measured
Time frame: 1 hour post-surgery
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.