This study evaluates the oxidative stress parameters in females with polycystic ovary syndrome (PCOS) and clinically healthy. Because of the fact that both oral disease included periodontitis and dental caries, and metabolic syndrome are associated with systemic inflammation, these two disorders may be linked through a common pathophysiologic pathway
Power analysis was performed with the G-Power software package to determine sample size. To cover possible data loss, 10% of a group were added to each group. Medical and dental examination will be performed both of control and experimental groups. Age and body mass index (BMI) will be recorded. In dental examination decayed, missing, or filled teeth (DMFT) index will be used according to World Health Organization (WHO 1997) criteria. All teeth were visually using the International Caries Detection and Assessment System (ICDAS-II). To analyze the correlation between oral health status and polycystic ovary syndrome linear regression test and for comparison of both the groups (case and control), two sample t test and chi square test were used.
Study Type
OBSERVATIONAL
Enrollment
206
3 to 5th day of the women's normal cycle, we will perform a routine gynecological examination, a basic vaginal ultrasound, a basal hormone profile evaluation
Recep Tayyip Erdogan University
Rize, Turkey (Türkiye)
FSH hormone ovarian morphology (PCOM)
follicle-stimulating hormone \[FSH\] is tested by blood sample 6.3\_24 mlu/ml
Time frame: 1 Day
luteinizing hormone
luteinizing hormone \[LH\] is tested by blood sample 1.68-15 U/L
Time frame: 1 day
dehydroepiandrosterone-sulfate
dehydroepiandrosterone-sulfate \[DHEA-S\] is tested by blood sample 65 to 380 µg/dL or 1.75 to 10.26 µmol/L
Time frame: 1 day
17-OH-progesterone
17-OH-progesterone is tested by blood sample 20-100 ng/dL
Time frame: 1 day
dental examination
All teeth were visually using the International Caries Detection and Assessment System (ICDAS-II). The chosen sites were recorded as: 0 = sound; 1. = first visible sign of noncavitated lesion seen only when the tooth is dried; 2. = visible noncavitated lesion seen when wet and dry; 3. = microcavitation in enamel; 4. = noncavitated lesion extending into dentine seen as an undermining shadow; 5. = small cavitated lesion with visible dentine: less than 50% of surface; 6. = large cavitated lesions with visible dentine in more than 50% of the surface.
Time frame: 1 Day
periodontal examination
A single calibrated examiner measured probing depth-PD, 0: healthy bleeding calculus 3:3.5-5.5 mm 4: over 5.5 mm
Time frame: 1 Day
Clinical attachment level
A single calibrated examiner measured clinical attachment level- CAL, 0: 0-3 mm 1:4-5 mm 2:6-8 mm 3:over 8mm 4: 9-11 mm 5: over 12 mm
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Time frame: 1 Day
Plaque Examination
The plaque amount is scored by using Silness\&Löe Plaque Index Each of the four surfaces of the teeth (buccal, lingual, mesial and distal) is given a score from 0-3. 0:no plaque 1. A film of plaque 2. soft deposit s within the gingival pocket 3. Abundance of soft matter within the gingival pocket. Calculation: Total scores of 6 (16, 12, 24, 36, 32, 44) teeth / No of surfaces examined Healthy = PI\<0.4. Mild = PI 0.4-1.0. Moderate = PI 1.1-2. Severe = PI\>2.
Time frame: 1 Day
Gingival Examination
0= Normal gingiva; 1. Mild inflammation 2. Moderate inflammation 3. Severe inflammation Calculation: Total scores/ no of surfaces examined 0.1-1:Mild gingivitis, 1.1-2:moderate gingivitis; 2.1-3:severe gingivitis
Time frame: 1 Day
Bleeding Examination
0: no bleeding 1: bleeding
Time frame: 1 Day