Association Between Mitochondrial DNA Content and Risk of Acute Leukemia

Air Force Military Medical University, China300 enrolled

Overview

Compelling epidemiological evidence indicates that alterations of mitochondrial DNA, including mutations and abnormal content of mitochondrial DNA (mtDNA), are associated with the initiation and development of acute lymphoblastic leukemia (ALL).The aim of this study was to explore association between mtDNA content in peripheral blood cells could be used as a risk predictor for ALL.

Study Type

OBSERVATIONAL

Enrollment

300

Conditions

Lymphoblastic Leukemia in Children

Interventions

Relative copy number of mitochondrial DNADIAGNOSTIC_TEST

Mitochondria are responsible for multiple cellular functions including regulation of energy production, modulation of oxidation-reduction status, generation of reactive oxygen species and apoptosis. Each mitochondrion possesses multiple copies of a mitochondrial genome comprised of independently replicating double stranded DNA (mtDNA).

Eligibility

Sex: ALLMax age: 16 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * 1.histological confirmed chronic lymphocytic leukemia; 2.preparing for chemotherapy; 3.no preoperative anticancer treatment. Exclusion Criteria: * 1.history of other malignancy; 2.blood transfusion within one month or prior bone marrow transplantation; 3.patients who reluctant to sign informed consent.

Locations (1)

Xiao-Fan Zhu

Tianjin, Tianjin Municipality, China

Outcomes

Primary Outcomes

Relative copy number of mitochondrial DNA

The ratio of mitochondrial DNA contents to hemoglobin contents was calculated for each sample from standard curves. After that, the ratio for each sample was normalized to a calibrator DNA in order to standardize between different runs, and then defined as the measurement of relative mtDNA contents.Relative expression of mtDNA were measured in young adults suffered from chronic lymphocytic leukemiawhen compared with control group.

Time frame: From date of admission until the date of discharging from hospital, assessed up to 5 days

Data from ClinicalTrials.gov

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