The aim of the study is to investigate the effect of rapeseed proteins on the postprandial metabolic response. Therefore, study participants will receive a meal rich in fat and carbohydrates with and without rapeseed protein or with soy protein that serves as control.
The current study is a monocentric, controlled intervention study that will be conducted as cross-over design. Three interventions are planned and participants will be invited to consume a test meal with added rapeseed protein isolate, a test meal with added soy protein isolate and a test meal without additional protein. The evening before, the subjects will receive a standardized dinner. After a 12-hour fasting overnight, a cannula will be placed. Blood samples will be taken before and after eating the test meal rich in carbohydrate and fat. The test meal consists of pasta with oily tomato sauce with either 25 g of rapeseed protein, 25 g of soy protein or no additional protein. A total of 11 blood samples will be collected over a period of 6 hours. In the meantime, the blood pressure and pulse will be measured repeatedly and information on satiety will be recorded. The interventions will be interrupted by a period of, at least, 2 weeks.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
20
25g rapeseed protein in comparison to 25g soy protein or no protein - dosage of 25 g per day
Martin-Luther-University Institute of Agriculture and Nutritional Science
Halle, Saxony-Anhalt, Germany
time course of glucose level
Impact of rapeseed protein on the postprandial blood glucose level
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
Cholesterol
Impact of rapeseed protein on the postprandial levels of Cholesterol
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
triglycerides
Impact of rapeseed protein on the postprandial levels of triglycerides
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
short chain fatty acids
Impact of rapeseed protein on the postprandial levels of short chain fatty acids
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
indole-3-propionate
Impact of rapeseed protein on the postprandial levels of indole-3-propionate
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
amino acids
Impact of rapeseed protein on the postprandial levels of amino acids
Time frame: 0,15,30,45,60,90,120 and 180 min after ingestion of testmeal rich in carbohydrates and fat
hormones (insulin, ghrelin, FGF23)
Impact of rapeseed protein on the postprandial levels of hormones (insulin, ghrelin, FGF23)
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Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
time course of urea level,
Impact of rapeseed protein on the postprandial levels of urea
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
minerals and blood hsCRP
Impact of rapeseed protein on the postprandial levels of minerals and blood hsCRP
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
systolic and diastolic blood pressures
time course: measurement of blood pressure in mm Hg
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
feeling of satiety by means of a questionnaire
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat
heart rate
time course: measurement of heart rate in beats per minute
Time frame: 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat