The Differences of Oocyte Maturation, Granulosa Cell Apoptosis Index Between Electroacupuncture And Sham Groups IVF

Indonesia University24 enrolled

Overview

Interventional studies compared electroacupuncture group versus sham electroacupuncture group to granulosa cell apoptosis index, oocyte maturation, GDF9 and BMP15 levels, and fertilization rate in in vitro fertilization program

The study was conducted at the Dr.Cipto Mangunkusumo National General Hospital, Indonesia in September - November 2018.The inclusion criteria are patients who underwent controlled ovarian stimulation with antagonist protocol, age 30-39 years, BMI \<29kg / m2. Exclusion criteria were not having a skin infection at acupuncture point and not using a pacemaker. Then patients were randomly divided into two groups: electroacupuncture and sham electroacupuncture. Electroacupuncture therapy was done 6 times at: 2 times before and 4 times during controlled ovarian stimulation.Apoptotic index of granulosa cells were examined by quantitative real time PCR techniques for BAX/BCL-2 expression.Oocyte maturation and fertilization rate output parameters are examined by competent embryologists after ovum pick up while GDF9 and BMP15 levels were examined by quantitative real time PCR techniques for GDF9 and BMP15 mRNA expression

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

TREATMENT

Masking

TRIPLE

Enrollment

Conditions

Acupuncture in Vitro Fertilization BCL2 Gene mRNA Overexpression Oocyte Maturation

Interventions

ElectroacupuncturePROCEDURE

Electroacupuncture therapy is done at the point of CV3 Zhongji, CV4 Guanyuan, and EXCA-1 Zigong with continuous wave, 2 Hz frequency for 30 minutes. Acupuncture manuals on endocrine ear points, GV20 Baihui, ST36 Zusanli, SP6 Sanyinjiao, BL57 Chengsan and KI3 Taixi for 30 minutes.

Eligibility

Sex: FEMALEMin age: 30 YearsMax age: 39 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * women underwent controlled ovarian stimulation with antagonist protocol * BMI \< 29 kg/m2 * willing and sign the inform consent * follow the research until completed Exclusion Criteria: * having skin infection at acupoint location * use a pacemaker

Locations (1)

Indonesian University

Jakarta Pusat, DKI Jakarta, Indonesia

Outcomes

Primary Outcomes

Index apoptotic (Ratio Bax/Bcl-2) of granulosa cells, GDF9 and BMP15

Bax/Bcl-2 is proapoptotic protein compare to antiapoptotic protein. GDF9 and BMP15 protein expression

Time frame: "up to 1 months"

Secondary Outcomes

oocyte maturation index

fertilization rate is number of fertilized oocytes compared with all total number oocyte which taken when ovum pick-up. oocyte maturation index is the number of mature oocyte (metaphase II oocyte) compare with all total number oocyte which taken when ovum pick-up

Time frame: "up to 1 months"

Fertilization rate

fertilization rate is number of fertilized oocytes compared with all total number oocyte which taken when ovum pick-up.

Time frame: "up to 1 months"

Data from ClinicalTrials.gov

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