To characterize the effects of three chocolate snacks on cognitive performance, subjective appetite, food intake control, and glycemic response in normal weight adults.
On 4 separate mornings, 7 days apart, 12 hours after an overnight fast, and 3 hours after a standardized breakfast of a cereal bar, orange juice and fruit cup, participants will consume isocaloric snacks of white chocolate, 2 types of dark chocolate or control (water), followed by an ad libitum pizza lunch 90 minutes later. Upon arrival, at baseline (0 minutes), participants will be asked to complete the motivation-to-eat, global vigor and subjective emotion visual analogue scales (VAS), a battery of cognitive performance tasks and have blood samples taken, as reported and validated in our previous studies. Over the 90 minute testing period, blood samples will be taken,the VAS questions will be completed and cognitive tasks will be completed. Cognitive performance assessments will include learning and memory, spatial memory, attention and processing speed, and executive functions. VAS measuring physical comfort is completed pre- and post-meal. Ad libitum food intake from a test meal will be measured.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
DOUBLE
Enrollment
26
Water
Experimental snack treatment
Experimental snack treatment
School of Nutrition, Ryerson University
Toronto, Ontario, Canada
Verbal declarative memory
One of six word lists composed of 15 words will be audio presented and participants will be asked to write them down. The word list will be presented 3 times and the participant will immediately write down words after each time the word list is presented. This test will take 5 minutes
Time frame: At 15 minutes
Change from baseline spatial memory
Assessed using a spatial pattern recognition test on the Membrain Application. There will be a grid of 9 boxes with circles randomly located inside each box. Participants will be asked to remember where circles are located in each box. This test is done 6 times at each time-point, with each test having different random locations of circles. Each test will take 20 seconds.
Time frame: At Baseline, 15, 45, and 75 minutes
Change in verbal declarative memory from the 15 minute time-point
Participants will be asked to recall and write down all the words from the word list presented at the 15 minute time-point. This test will take 2 minutes.
Time frame: At 45 and 75 minutes
Change from baseline executive function
Will be assessed the Stroop task via the Membrain application. Participants will be presented with a list of words presented in colors that match the word (congruent, the word 'red' presented in red) or colors that do not match the word (incongruent, the word 'red' presented in blue). Participants will be asked to identify the color of the word, not the word itself. The task is scored for the number of correct and incorrect colors identified, as well as total time to completion. This test will be at each time-point and will take 1 minute to complete.
Time frame: At Baseline, 15, 45, and 75 minutes
Change from baseline working memory and executive function
A random 3 digit number will appear on the screen of the Membrain Application and the participant will have to continually subtract 3 from this number. At each time-point, participants will also complete the same test, except will subtract 7 continuously. This is a 2 minute test.
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Experimental snack treatment
Time frame: At Baseline, 15, 45, and 75 minutes
Change from baseline attention and reaction time
Using the Membrain Application, Aa continuous string of single digit numbers appear on the screen, one at a time (each appearing for about one second). After seeing three consecutive ascending odd numbers, participants click the 'odd' button. When they see three consecutive, ascending even numbers, they click the 'even' button. When they see the number "1", they click the 'tertiary' button. This test is 5 minutes in length.
Time frame: At Baseline, 15, 45, and 75 minutes
Change from baseline subjective average emotions
Measured using 100 point visual analogue scales. Participants mark on this line to describe their feelings.
Time frame: At Baseline, 15, 30, 45, 60, 75 and 90 minutes
Change from baseline global vigor
Measured using 100 point visual analogue scales. Participants mark on this line to describe their feelings.
Time frame: At Baseline, 15, 30, 45, 60, 75 and 90 minutes
Change from baseline subjective average appetite
Measured using 100 point visual analogue scales. Participants mark on this line to describe their feelings.
Time frame: At Baseline, 15, 30, 45, 60, 75 and 90 minutes
Change from baseline blood glucose concentration
Blood glucose concentration (mmol/L) will be measured in whole blood using YSI 2300 STAT PLUS (YSI Incorporated, Yellow Springs, OH)
Time frame: At Baseline, 15, 30, 45, 60, 75 and 90 minutes
Food intake
After other measures at the 90 minute time-point, participants will have lunch and food intake will be determined by weighing the meal before and after serving. The net weight of the test meal will be converted to kilocalories
Time frame: At 90 minutes