The overall aim of this study is to investigate how dairy products with different structures and textures affect lipid absorption kinetics in the acute postprandial period via blood lipid biochemistry. The study will be conducted as a randomized acute cross-over meal study. Apparently healthy men will be recruited in the study. They will on 4 test days consume the 4 dairy products and blood will be drawn the following 8 hours.
The overall aim of this study is to investigate how dairy products with similar nutrient content but different structures and textures affect lipid absorption kinetics in the acute postprandial period via blood lipid biochemistry, as well as nuclear magnetic resonance (NMR) and liquid chromatography mass spectrometry (LCMS)-based metabolomics. The study will be conducted as a randomized acute cross-over meal study. On four different test days (with a minimum of 2 weeks washout period) four dairy products with similar nutrient composition but different structures or textures will be tested. During each meal test a single dairy product (+ bread and water) is served and postprandial blood samples are collected the following 8 hours. Subjective appetite measurements (VAS) are filled in during the 8-hour period and an ad libitum meal is served in the end of the day. 25 apparently healthy men (weight stable, 18-40 years, 18.5-24.9 kg/m2) will be recruited.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
SINGLE
Enrollment
25
The reference product is a commercial matured cheddar cheese
A blended and homogenized cheddar cheese (a spread) which, compared to the reference, has a minimal protein network and has smaller fat droplet
An anolog milk (i.e. milk with a cheese-like nutritional composition) which, compared to the reference, does not contain a protein network
Department of Nutrition, Exercise and Sports
Frederiksberg, Denmark
Triglyceride
The sample size is calculated on this endpoint. Fasting and postprandial concentrations of triglycerides (mmol/L) are measured after intake of a test meal.
Time frame: 0-8 hour
Apolipoprotein B48
Co-primary endpoint. Fasting and postprandial concentrations of apolipoprotein B48 are measured after intake of a test meal.
Time frame: 0-8 hour
Cholesterol (total, LDL and HDL cholesterol)
Fasting and postprandial concentrations of cholesterol (mmol/L) are measured after intake of a test meal.
Time frame: 0-8 hour
Free Fatty Acid (FFA)
Fasting and postprandial concentrations of FFA (umol/L) are measured after intake of a test meal.
Time frame: 0-8 hour
Insulin
Fasting and postprandial concentrations of insulin (pmol/L) are measured after intake of a test meal.
Time frame: 0-8 hour
Glucose
Fasting and postprandial concentrations of glucose (mmol/L) are measured after intake of a test meal.
Time frame: 0-8 hour
Apolipoproteins
Fasting and postprandial concentrations of apolipoproteins (e.g. B100) are measured after intake of a test meal.
Time frame: 0-8 hour
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This analog cheese (a gel/yoghurt) is produced from the analog milk which, compared to the reference, has a protein network, however it is a network that differs from the reference