The aim of this study is to analyse the efficacy of MACS technique for the selection of spermatozoa and its effects on implantation, pregnancy and miscarriage rate. This study will be performed by two IVI clinics (IVI Sevilla and IVI Madrid) with the purpose of finding new techniques that allow us to better select spermatozoa and improve the results of assisted reproduction techniques.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
DOUBLE
Enrollment
130
MACS is a technique that allow us to tag and detect apoptotic spermatozoa. These apoptotic spermatozoa have phosphatidylserine in their membranes, so we add magnetic beads with Annexin V, which binds specifically to phosphatidylserine. The spermatozoa are then tagged and can be retained in a magnetic field, allowing us to collect just the non-apoptotic spermatozoa.
IVI Sevilla
Seville, Spain
Pregnancy rate
The number of pregnancies confirmed by ultrasound divided by the number of artificial insemination cycles. Pregnancy is confirmed by ultrasound examination.
Time frame: Ultrasound examination is performed 5 weeks after artificial insemination. Pregnancy rate is calculated during the phase of data analysis.
Implantation rate
The number of positive results of beta human chorionic gonadotropin (hCG) test divided by the number of artificial insemination cycles. Implantation is confirmed through the analysis of beta hCG in blood.
Time frame: The beta hCG analysis is performed 15 days after artificial insemination. Implantation rate is calculated during the phase of data analysis.
Live birth rate
The number of live births divided by the number of artificial insemination cycles
Time frame: Live births are assessed at delivery (end of pregnancy period). Live birth rate is calculated during the phase of data analysis
Sperm cell concentration
Concentration of sperm cells in sperm sample before and after applying the MACS technique
Time frame: This parameter is assessed when preparing the sample for artificial insemination (one hour before artificial insemination).
Sperm cell motility
Proportion of sperm cells with progressive motility (according to World Health Organisation 2010 criteria) before and after applying the MACS technique
Time frame: This parameter is assessed when preparing the sample for artificial insemination (one hour before artificial insemination).
Sperm cell morphology
Proportion of sperm cells with normal morphology (according to World Health Organisation 2010 criteria) before and after applying the MACS technique
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Time frame: This parameter is assessed when preparing the sample for artificial insemination (one hour before artificial insemination).
Sperm cells vitality
Proportion of live sperm cells before and after applying the MACS technique
Time frame: This parameter is assessed when preparing the sample for artificial insemination (one hour before artificial insemination).
Sperm sample volume
The volume of sperm obtained after masturbation
Time frame: This parameter is assessed when preparing the sample for artificial insemination (one hour before artificial insemination).