This study was conducted to evaluate and compare cryo-survival, re-expansion, and implantation rates of Day 5/6 human blastocysts that were vitrified with home-made media (HM) according to (Shady grove protocol) and warmed with gradual Thawing HM according to (Kuwayama protocol)with Global Fast Freeze/Thaw Kits (LifeGlobal, Canada).
Group 1 blastocysts were vitrified with the Vit HM according to shady grove protocol using open system on cryotopvit. device and thawed with gradual thawing technique using HM according to Kuwayama protocol. Group 2 blastocysts were cryopreserved with the Global Fast Freeze Kitusing open system on cryotopvit. device and thawed using Gobal fast thaw kit. Blastocysts were subsequently cultured for 6-8 h, assessed for survival and expansion, then transferred and the percentages of Positive hCG, clinical pregnancy, implantation, multiple pregnancy and abortion were recorded.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
74
home made vitrification was made and prepared for vitrification of blastocysts then thawing with a prepared thawed media too
commercially available vitrification \& thawing kit were made and prepared for vitrification of blastocysts then thawing with a prepared thawed media too
blastocyst immediate survival
The effect of Vitrification protocol on the blastocyst immediate survival
Time frame: 0 hour at time of thawing
pregnancy rate
clinical pregnancy rate in these cases
Time frame: 2 weeks after transfer
live birth rate
live birth rate of positive pregnancy cases
Time frame: 9 months
Blastocyst survival at 6 hours
The effect of Vitrification protocol on the blastocyst survival after 6 hours
Time frame: 6 hours after thawing
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