Neurogenic Inflammation in Peri-implant and Periodontal Diseases

Ondokuz Mayıs University39 enrolled

Overview

Regulatory effects of some neuropeptides substance-P (SP), neurokinin-A (NKA), calcitonin gene-linked peptide (CGRP) and neuropeptide-Y (NPY) )on inflammatory responses in periodontal disease has been described; however, the impact of neuropeptide levels are not clearly defined in healthy and diseased peri-implant tissues.ın order to evaluate the situation, thirty-nine implants that have been loaded over the past 12 months and their symmetrically matching teeth were evaluated using a split-mouth study design. Clinical periodontal examinations included the Silness-Löe plaque index, Löe-Silness gingival index, bleeding on probing, probing pocket depth and clinical attachment level parameters were determined. Gingival crevicular fluid (GCF)/Periimplant sulcular fluid (PISF) samples were collected, and the levels of the neuropeptides were determined by enzyme-linked immunosorbent assay. Correlations between GCF/PISF neuropeptide levels and the clinical examination parameters were evaluated in the peri-implant/periodontal soft tissues.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Neurogenic Inflammation Periimplant Diseases Periodontal Diseases Gingival Crevicular Fluid Periimplant Sulcular Fluid

Interventions

gingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collectionDIAGNOSTIC_TEST

GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.

Eligibility

Sex: MALEMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: The main inclusion criteria for patient selection were as follows: * Compliance with systematic maintenance care; * Adult, non-smoker, systemically healthy * Not using any medicament for the last 6 months ; * Partially dentated patient treated with implant-supported metal ceramic full crowns and/or fixed partial dentures * As the study was split-mouth designed, in each individual at least one implant and one matching tooth in the same mouth symmetrically representing the same tooth group (premolars/molars); * A single type of metal ceramic implant restoration that is functional for at least 1 year; and a matching tooth with no dental or endodontic restorations. Exclusion Criteria: * Medical history of cancer, rheumatoid arthritis, diabetes mellitus, or cardiovascular disease and any other systemic disease affecting lipid metabolism (i.e. impaired glucose tolerance, metabolic syndrome); * Compromised immune system; * Pregnancy, menopause, or lactation; * Ongoing drug therapy that might affect the clinical characteristics of periodontitis and lipid metabolism; * Dental treatment during the 6 months prior to data collection.

Outcomes

Primary Outcomes

Gingival crevicular fluid/peri-implant sulcular fluid Level of Substance-P

Substance-P (SP) is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.

Time frame: 8-10 am on the day following periodontal status assessment.

Gingival crevicular fluid/peri-implant sulcular fluid Level of neurokinin-A

Neurokinin-A is a neuropeptide of the tachykinin family and marker of neurogenic inflammation.

Time frame: 8-10 am on the day following periodontal status assessment.

Gingival crevicular fluid/peri-implant sulcular fluid Level of Calcitonin gene-related peptide

Calcitonin gene-related peptide (CGRP), another neuropeptide derived from peptidergic nerves and a marker of neurogenic inflammation.

Time frame: 8-10 am on the day following periodontal status assessment.