A total 180 semen samples from couples diagnosed with unexplained infertility were SDF index tested , then total 120 semen samples of patients' husbands with abnormal SDF index were randomly divided and then processed by swim up, sperm gradient centrifugation and Zeta methods. SDF and ICSI outcomes are monitored after semen processing.
A total 180 semen samples from couples diagnosed with unexplained infertility were SDF index tested , then total 120 semen samples of patients' husbands with abnormal SDF index were randomly divided and then processed by swim up (G1/ n=40), sperm gradient centrifugation (G2/ n=40) and Zeta (G3/ n=40) methods. SDF test was assessed by Halosperm kit, whereas sperm morphology was assessed by spermac stain according to strict criteria. Fertilization, division, blastulation, implantation and pregnancy rates will be tabulated and statistically tested.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
TRIPLE
Enrollment
20
different sperm processing method for selcting most proper sperm for ICSI
Ahmed Saad
Banhā, Qalyubia Governorate, Egypt
Sperm DNA fragmentation
measure sperm integrity by halosperm assay
Time frame: 30 minutes
Oocyte fertilization rate
no. of oocytes fertilized /total no. of oocytes
Time frame: day 1
Blastulation rate
no of embryos blastulated / total no. of embryos
Time frame: day 5
pregnancy rate
no of pregnant cases\\ all no. of cases
Time frame: 15 days
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