Follicular Activation by Fragmentation of Ovarian Tissue

Inclusion Criteria: 1. previous ≥ 2 poor ovarian responds (≤ 3 retrieved oocytes) in an antagonist cycle with a minimum of 225 IU gonadotropins a day or corifollitropin 150 mcg) in patients \>35 i \<40 years 2. previous cycle with ≤ 3 retrieved oocytes) in an antagonist cycle with a minimum of 225 IU gonadotropins a day or corifollitropin 150 mcg) in patients \>35 i \<40 years with antral follicle count (AFC) ≤ 5 i/ili antiM¸ulelrian hormone (AMH) ≤ 6.5pmol/L 3. premature ovarian insufficiency according to European Society of Human Reproduction and Embryology (ESHRE) development group, 2015 in patients 18-40 years 4. undetectable AMH serum levels and follitropin (FSH) \>35 IU/ml in two serum samples separated by at least 4 weeks Exclusion Criteria: 1. clinical/ultrasonographical signs of endometriosis 2. previous ovarian/pelvic surgery 3. previous gonadotoxic treatment 4. genito-urinary tract malformations 5. extreme male infertility ( \<10 000 progressively motile sperms in the semen sample) 6. inform consent unsigned -