Lifestyle factors, such as diet, physical activity and sleep, are associated with the development of many chronic diseases. The objective of The Manitoba Personalized Lifestyle Research (TMPLR) study is to understand how these lifestyle factors interact with each other and additional factors, such as an individual's genetics and gut microbiome, to influence health. This is an exploratory cross-sectional observational cohort study of adults, with extensive phenotyping by objective health and lifestyle assessments, and retrospective assessment of early life experiences, with retrospective and prospective utilization of secondary data from administrative health records. A planned non-random convenience sample of 840 Manitobans aged 30-46 recruited from the general population, stratified by sex (equal males and females), body mass index (BMI; 60% of participants with a BMI \>25 kg/m2), and geography (25% from rural areas,). These stratifications were selected based on Manitoba demographics. Body composition and bone density will be measured by dual energy x-ray absorptiometry. Blood pressure, pulse wave velocity, and augmentation index will be measured on two consecutive days. Chronic disease risk biomarkers will be measured in blood and urine samples. DNA will be extracted for genetic analysis. A fecal sample will be collected for microbiome analysis.
Lifestyle factors, such as diet, physical activity and sleep, are associated with the development of many chronic diseases. The objective of The Manitoba Personalized Lifestyle Research (TMPLR) study is to understand how these lifestyle factors interact with each other and additional factors, such as an individual's genetics and gut microbiome, to influence health. This is an exploratory cross-sectional observational cohort study of adults, with extensive phenotyping by objective health and lifestyle assessments, and retrospective assessment of early life experiences, with retrospective and prospective utilization of secondary data from administrative health records. A planned non-random convenience sample of 840 Manitobans aged 30-46 recruited from the general population, stratified by sex (equal males and females), body mass index (BMI; 60% of participants with a BMI \>25 kg/m2), and geography (25% from rural areas,). These stratifications were selected based on Manitoba demographics. Lifestyle factors assessed will include dietary pattern, physical activity, cardiovascular fitness and sleep. Additional factors such as medical history, socio-economic status, alcohol and tobacco consumption, cognition, stress and anxiety, and early life experiences will also be documented. A maternal survey will be performed. Body composition and bone density will be measured by dual energy x-ray absorptiometry. Blood pressure, pulse wave velocity, and augmentation index will be measured on two consecutive days. Chronic disease risk biomarkers will be measured in blood and urine samples. DNA will be extracted for genetic analysis. A fecal sample will be collected for microbiome analysis. Participants may provide their Manitoba Personal Health Information Number (PHIN) to link their study data with administrative health records.Lifestyle factors, such as diet, physical activity and sleep, are associated with the development of many chronic diseases. The objective of The Manitoba Personalized Lifestyle Research (TMPLR) study is to understand how these lifestyle factors interact with each other and additional factors, such as an individual's genetics and gut microbiome, to influence health. This is an exploratory cross-sectional observational cohort study of adults, with extensive phenotyping by objective health and lifestyle assessments, and retrospective assessment of early life experiences, with retrospective and prospective utilization of secondary data from administrative health records. A planned non-random convenience sample of 840 Manitobans aged 30-46 recruited from the general population, stratified by sex (equal males and females), body mass index (BMI; 60% of participants with a BMI \>25 kg/m2), and geography (25% from rural areas,). These stratifications were selected based on Manitoba demographics. Lifestyle factors assessed will include dietary pattern, physical activity, cardiovascular fitness and sleep. Additional factors such as medical history, socio-economic status, alcohol and tobacco consumption, cognition, stress and anxiety, and early life experiences will also be documented. A maternal survey will be performed. Body composition and bone density will be measured by dual energy x-ray absorptiometry. Blood pressure, pulse wave velocity, and augmentation index will be measured on two consecutive days. Chronic disease risk biomarkers will be measured in blood and urine samples. DNA will be extracted for genetic analysis. A fecal sample will be collected for microbiome analysis. Participants may provide their Manitoba Personal Health Information Number (PHIN) to link their study data with administrative health records.
Study Type
OBSERVATIONAL
Enrollment
840
University of Manitoba
Winnipeg, Manitoba, Canada
Percent fat mass
Measured using dual-energy X-ray absorptiometry
Time frame: Baseline
Percent lean mass
Measured using dual-energy X-ray absorptiometry
Time frame: Baseline
Bone mineral density
Measured using dual-energy X-ray absorptiometry, calculated as (mg/cm\^2)
Time frame: Baseline
(ASA24) dietary assessment tool
(ASA24) dietary assessment tool
Time frame: Baseline
Food energy intake
Assessed using dietary history questionnaire
Time frame: Baseline
Macronutrient intake
Assessed using dietary history questionnaire
Time frame: Baseline
Micronutrient intake
Assessed using dietary history questionnaire
Time frame: Baseline
Dietary restraint
Score 0-20 from Three-Factor Eating Questionnaire
Time frame: Baseline
Dietary disinhibition
Score 0-16 from Three-Factor Eating Questionnaire
Time frame: Baseline
Hunger
Score 0-14 from Three-Factor Eating Questionnaire
Time frame: Baseline
Pittsburgh sleep quality index
Pittsburgh sleep quality index
Time frame: Baseline
Montreal cognitive assessment questionnaire
Total score of 30; minimum score 0; maximum score of 30
Time frame: Baseline
Frailty status, as determined by the Modified Fried Criteria
Patients meeting the criteria for ≥ 3 of the following 7 criteria are deemed frail. 1. Slowness - After two trials of a 5 m walk, average time\>6 s 2. Weakness - After three grip strength measurements with each hand, maximum value 30 kg if male or 20 kg if female 3. Weight loss - Self-reported weight loss \>4.5 kg (10 lbs) or \>5% body weight in past 12 months 4. Exhaustion - Two-item Center for Epidemiologic Studies Depression (CES-D) scale 1 out of 2 5. Depression - Five-item Geriatric Depression Scale (5-GDS) 2 out of 5 6. Low physical activity - Paffenbarger Physical Activity Index \<383 kcal per week if male or \< 270 kcal per week if female 7. Cognitive impairment - Montreal Cognitive Assessment (MoCA) score \<26 out of 30
Time frame: Baseline
Childhood retrospective circumstances questionnaire (adapted from the US Panel Study on Income Dynamics)
Childhood retrospective circumstances questionnaire (adapted from the US Panel Study
Time frame: Baseline
Weight
Participants change into lightweight scrub tops and bottoms, with shoes removed, to the nearest 0.1 kg using a digital calibrated floor scale
Time frame: Baseline
Height
Measured, without shoes, to the nearest 0.1 cm using a stadiometer
Time frame: Baseline
Body mass index (BMI)
Calculated as kg/m\^2
Time frame: Baseline
Waist circumference
Measured in triplicate, to the nearest 0.1 cm at the umbilicus, between the last rib and the iliac crest using a fibreglass tape measure
Time frame: Baseline
Hip circumference
Measured in triplicate at the widest portion of the buttocks and hips using a fibreglass tape measure
Time frame: Baseline
Blood pressure
Systolic and diastolic blood pressures are measured in triplicate, on the non-dominant arm in a sitting position using a validated oscillometric blood pressure monitor
Time frame: Baseline
Pulse wave velocity
Measured on the non-dominant arm in a sitting position using a Mobil-O-Graph PWA Monitor on two consecutive days
Time frame: Day 1 and Day 2
Augmentation index
Measured on the non-dominant arm in a sitting position using a Mobil-O-Graph PWA Monitor on two consecutive days
Time frame: Day 1 and Day 2
Blood total cholesterol
Blood total cholesterol
Time frame: Baseline
Blood low-density lipoprotein cholesterol
Blood low-density lipoprotein cholesterol
Time frame: Baseline
Blood high-density lipoprotein cholesterol
Blood high-density lipoprotein cholesterol
Time frame: Baseline
Blood triglycerides
Blood triglycerides
Time frame: Baseline
Blood glucose
Blood glucose
Time frame: Baseline
Blood insulin
Blood insulin
Time frame: Baseline
Blood urea
Blood urea
Time frame: Baseline
Blood creatinine
Blood creatinine
Time frame: Baseline
Blood high sensitivity C-reactive protein
Blood high sensitivity C-reactive protein
Time frame: Baseline
Blood aspartate aminotransferase
Blood aspartate aminotransferase
Time frame: Baseline
Blood alanine aminotransferase
Blood alanine aminotransferase
Time frame: Baseline
Blood T regulatory cells
Blood T regulatory cells
Time frame: Baseline
Blood leptin
Blood leptin
Time frame: Baseline
Blood glucagon
Blood glucagon
Time frame: Baseline
Urinary melatonin
Urinary melatonin
Time frame: Baseline
Red blood cell fatty acids
Red blood cell fatty acids
Time frame: Baseline
Plasma fatty acids
Plasma fatty acids
Time frame: Baseline
Blood non-cholesterol sterols
Blood non-cholesterol sterols
Time frame: Baseline
Blood vitamin C levels
Blood vitamin C levels
Time frame: Baseline
Fractional cholesterol synthesis rate
Fractional cholesterol synthesis rate
Time frame: Baseline
Triglyceride synthesis rate
Triglyceride synthesis rate
Time frame: Baseline
Gastrointestinal microbiome
Microbiome 16S RNA sequencing
Time frame: Baseline
Physical activity level
Assessed using accelerometers over a period of 1 week
Time frame: 1 week
Muscle strength
Measured using a hand grip dynamometer
Time frame: Baseline
Cardiorespiratory fitness
Assessed using a metabolic cart to measure oxygen consumption (VO2; mL O2/kg body weight/minute) during a submaximal YMCA bike protocol performed to 85% of age predicted heart rate max
Time frame: Baseline
Functional walking ability
Assessed using a 5-meter gait speed test
Time frame: Baseline
Sleep
Average hours of sleep per night; measured using accelerometers over a period of 1 week
Time frame: 1 week
Identification and characterization of susceptibility genes for negative outcomes of lifestyle factors
This is meant to be achieved through a Genome Wide Association Study (GWAS) of Single Nucleotide Polymorphism (SNPs) associated with the measures of the lifestyle factors assessed in the TMPLR cohort, as listed in "outcome measures". Whole genome genotyping will be conducted with a state of the art genotyping array.
Time frame: Baseline
Identification and characterization of susceptibility genes for negative outcomes of lifestyle factors.
This is meant to be achieved targeted resequencing of target genes know to effect the metabolic traits assessed. This will aid the detection rare of Single Nucleotide Variations (SNVs) impacting the genes functions. Targeted re-sequencing will be conducted with a state of the art methodology.
Time frame: Baseline
Early life experiences by self report
Assessed using the childhood retrospective circumstances questionnaire (adapted from the US Panel Study on Income Dynamics
Time frame: Baseline
Early life experiences by maternal report
Assessed using a mother's questionnaire (adapted from the Nurses' Health Study)
Time frame: Baseline
Early life health conditions from linked administrative health records, from the Manitoba Population Healthy Research Data Repository
Examples of health records include: prescriptions, physician diagnoses and hospital discharge abstracts
Time frame: Baseline
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