Impact of High-fat Meals Varying in Fatty Acid Composition on Adipose and Systemic Metabolic-inflammatory Responses

Inclusion Criteria: * 18-50 years * BMI = 25-40 kg/m2 * Male or Female * Waist circumference \>94 cm (men) and \>80cm (women)\* * Physically active (\> 3 x 30 min moderate intensity exercise per week) * Systolic blood pressure \< 160 mmHg and diastolic blood pressure \< 100 mmHg * No cardiometabolic (e.g. heart disease, high blood pressure, type 2 diabetes) or inflammatory illness * NOTE: If waist circumference falls below 94 cm for men or 80cm for women but BMI is \>25 kg/m2 volunteers may still be recruited at the PI's discretion. Exclusion Criteria: * Smoker * Previous diagnosis of anaemia * Women who are pregnant or lactating * Taking medication known to interfere with study outcomes (e.g. treatment for hyperlidaemia, hypertension, inflammation or hypercoagulation) or prescribed antibiotics within the last 3 months * Taking nutritional supplements known to interfere with study outcomes (e.g. fish oil or evening primrose oil) * Unstable weight history (\>3 kg loss or gain in the previous 3 months) * An allergy to lidocaine * Those with known or suspected food intolerances, allergies or hypersensitivity to any components of the meal (e.g. lactose/wheat intolerance) * Alcohol consumption \>28 units per week for a man or \>21 units per week for a woman * Any other unusual medical history or diet and lifestyle habits or practices that would preclude volunteers from participating in a dietary intervention or metabolic study * Parallel participation in another intervention study