This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
13
Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).
Participants will exercise on a recumbent bike placed on flat ground (CTL).
Queen's University
Kingston, Ontario, Canada
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
Fold-change in PGC-1α mRNA expression
Time frame: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions
Catecholamine concentrations
Catecholamine concentrations in venous blood
Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)
Fold change in p38 MAPK phosphorylation
Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
Total and phosphorylated acetyl coenzyme A carboxylase (ACC)
Fold change in ACC phosphorylation
Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
Total and phosphorylated protein kinase A (PKA)
Fold change in PKA phosphorylation
Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
Acetylated p53
Fold change in acetylated p53
Time frame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
Blood lactate
Average \[lactate\] between conditions
Time frame: During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions
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Heart rate (HR)
Average HR between conditions
Time frame: During both exercise sessions
Revolutions per minute (RPM)
Average RPM between conditions
Time frame: During both exercise sessions
Ratings of perceived exertion (RPE)
Average RPE between conditions
Time frame: During both exercise sessions
Oxygenated and deoxygenated hemoglobin
Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values
Time frame: During both exercise sessions and up to 10 minutes after (for cuff occlusion)