Relationships Among FVIII, t-PA/PAI-1, and MMP-9 Levels and Intracranial Hemorrhage Complications After Thrombolysis With Alteplase in Patients With Acute Ischemic Stroke: Protocol for a Multicenter Retrospective Study

Inclusion Criteria: * Diagnosis of ischemic stroke and within the time window of thrombolysis (Onset of symptoms no more than 4.5 hours ) * 18 y age 85 y• * Symptoms of neurological deficits caused by cerebral infarction * Informed consent form to confirm thrombolytic therapy have signed by patients or their family. Exclusion Criteria: * A history of major head trauma or stroke in the past 3 months * Suspicious subarachnoid hemorrhage * Arterial puncture at noncompressible site in the last week * History of intracranial hemorrhage * Intracranial neoplasm, arteriovenous malformation, or aneurysm * Recent intracranial or intraspinal surgery * Elevated blood pressure (systolic \>185 mm Hg or diastolic \>110 mm Hg) * Active internal bleeding or Acute bleeding diathesis * Platelet count \<100×109·L-1 * Heparin received within 48 h resulting in aPTT above the upper limit of normal * Current use of anticoagulant with INR \>1.7 or PT \>15 s * Current use of thrombin inhibitors or factor Xa inhibitors * Abnormal laboratory tests (eg, aPTT, INR, platelet count, ECT, TT, or appropriate factor Xa activity assays) * Blood glucose concentration \> 2.7 mmol/L * CT demonstrates multilobar infarction (hypodensity \>1/3 cerebral hemisphere) * Mild cerebral infarction or rapid improvement of symptoms of cerebral infarction * Symptoms of neurological impairment after seizures * Major surgery or severe trauma in the past 2 weeks * Gastrointestinal or urinary bleeding in the past 3 weeks * History of myocardial infarction in the past 3 months * Combine diseases that may affect outcomes,such as Anemia, hemophilia * History of autoimmune disease or organ transplantation * Pregnancy or breastfeeding women