The reference technique for the conservation of gametes is storage in liquid nitrogen but new vats of nitrogen vapor (storage over liquid nitrogen) or in dry phase (storage in an insulated compartment of liquid nitrogen in a tank Liquid nitrogen) also allow the storage of flakes. The purpose of this work is to evaluate the dry-phase cryopreservation technique of liquid nitrogen compared with liquid-phase storage, depending on the duration of cryopreservation.
Objective: To evaluate the effects of cryopreserved sperm in dry and liquid phase nitrogen at 3 and 6 month on sperm numeration, motility, vitality, morphology, acrosomal integrity and DNA fragmentation. Design: Experimental study, investigator was blinded to the type of Cryopreservation. Patient(s): Semen samples were collected from patients who came in laboratory for semen analysis Intervention: Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature.
Study Type
OBSERVATIONAL
Enrollment
52
Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature
Chu Clermont-Ferrand
Clermont-Ferrand, France
Sperm DNA integrity
the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA
Time frame: at 3 months
Sperm DNA integrity
the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA
Time frame: at 6 months
Sperm parameter post cryopreservation
The Sperm motility will be measured according to WHO recommendations
Time frame: at 3 and 6 months
Lab parameter post cryopreservation
The Sperm motility will be measured according to WHO recommendations
Time frame: at 3 and 6 months
Spermatic DNA Compaction
The spermatic DNA Compaction will be determinated by chromomycine A3 labelling (CMA3). A sperm is good when it has at least 30% of positive spermatozoa to CMA3 assay
Time frame: at 3 and 6 months
Acrosomal integrity
The acrosomal integrity will be determinated by PSA-FITC labelling.
Time frame: at 3 and 6 months
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.