Neutrophil Extracellular Traps Mediate Antiphospholipid Antibody-induced Pregnancy Loss

Shanghai First Maternity and Infant Hospital100 enrolled

Overview

1. Establish a multi-platform detection system for neutrophil extracellular traps in blood and tissues, and evaluate the detection performance; 2. Comparing the expression levels of neutrophil extracellular traps in peripheral blood and decidua of patients with antiphospholipid antibody-induced pregnancy loss and normal control group; 3. Comparing the expression levels of neutrophil extracellular traps in peripheral blood and decidua of mice with antiphospholipid antibody-induced pregnancy loss and normal control group; 4. After treatment of antiphospholipid antibody-induced mouse abortion model with metformin,the effect of metformin on embryo loss rate and viable embryo body weight was observed.

Study Type

OBSERVATIONAL

Enrollment

100

Conditions

Neutrophil Extracellular Traps; Antiphospholipid Antibody-induced Pregnancy Loss

Eligibility

Sex: FEMALEMin age: 25 YearsMax age: 41 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Clinical diagnosis of Recurrent spontaneous abortion * Clinical diagnosis of Antiphospholipid syndrome Exclusion Criteria: * Other autoimmune diseases * Hematopoietic system diseases * Cardiovascular system diseases * Gastrointestinal system diseases * Urinary system diseases * Nervous system or mental diseases * Have taken other drugs recently

Locations (1)

Outcomes

Primary Outcomes

NETs level

Neutrophil extracellular traps (NETs) are networks of extracellular fibers, primarily composed of DNA from neutrophils, which bind pathogens.

Time frame: through study completion, an average of 8 months

Secondary Outcomes

complete blood count (CBC)

CBC is a blood panel requested by a doctor or other medical professional that gives information about the cells in a patient's blood, such as the cell count for each blood cell type and the concentrations of hemoglobin.