Anorexia nervosa is an eating disorder whose symptomatology induces a modification of the intestinal microbiota. To date, studies have shown variable profiles without linking them to metabolic and neuropsychological energy phenotyping. This intestinal dysbiosis could be involved in the maintenance of the disorders. Bidirectional communication channels exist between the microbiota, the intestine and the brain. Anomalies in these pathways could explain the impact of the microbiota on the pathophysiology of anorexia nervosa. Therapeutic interventions would then be possible to restore the microbiota in anorexia nervosa and influence the treatment of this disease. This study aims to explore the hypothesis of disruption of the microbiota-intestinal-brain axis transversely and measuring the intestinal microbiota, urinary metabolome, biological factors nutritional, immunological and physiological plasma plasma of the intestine, and finally, the psychological dimensions characteristic of anorexia nervosa.
Study Type
OBSERVATIONAL
12 ml sample
20 g sample
18 ml sample
* Go-nogo * flexibility * food stroop * implicit and explicit evaluation * Child Depression Inventory * Hamilton Depression Rating Scale
Chu Saint-Etienne
Saint-Etienne, France
determination of bacterial microbiological profile by 16s sequencing of stool samples
Time frame: at inclusion
Determination of the metabolomic profile
evaluated by mass spectrometry (MS/MS) of urine
Time frame: at inclusion
Neurocognitive evaluation
evaluated with questionnaire go-nogo
Time frame: at 2 months
Neurocognitive evaluation
evaluated with questionnaire of flexibility
Time frame: at 2 months
Neurocognitive evaluation
evaluated with questionnaire : food troop
Time frame: at 2 months
Neurocognitive evaluation
evaluated with questionnaires : implicit and explicit evaluation.
Time frame: at 2 months
Psychometric scaling for thymic evaluation
evaluated with questionnaire : Child Depression Inventory
Time frame: at 2 months
Psychometric scaling for thymic evaluation
evaluated with questionnaire : Hamilton Depression Rating Scale
Time frame: at 2 months
Determination of the plasma profile of intestinal physiology markers and immunological markers
Intestinal permeability markers: ASCA, antiglycan, anti GP2 IgG/IgA, calprotectin
Time frame: at inclusion
Determination of the plasma profile of intestinal physiology markers and immunological markers
immunoglobulin repertoire: A and M, inflammatory molecules: C-reactive protein
Time frame: at inclusion
Determination of the plasma profile of intestinal physiology markers and immunological markers
circulating interleukins of the Th17 profile: IL17, IL23, IL6, IL1β, IL 22, IL21, IL33, IL31, TNFα, IFNγ, IL9, IL10, IL4.
Time frame: at inclusion
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