The purpose of this study is to examine whether a low carbohydrate diet as compared to simple calorie restriction decreases hepatic lipid content and changes hepatic mitochondrial flux in humans.
In this study, the investigators will examine whether a low carbohydrate diet as compared to simple calorie restriction will change hepatic lipid content and mitochondrial flux in humans. Before and after a low carbohydrate diet and a simple calorie restriction intervention, rates of hepatic mitochondrial flux will be assessed using positional isotopomer analysis of 3-13C-lactate during a 3 hour basal period. Rates of whole body ketogenesis will be determined using \[13C4\]β-hydroxybutyrate, and whole body glucose metabolism will be measured using \[2H7\]glucose. Body composition will be determined with bioelectrical impedance; hepatic lipid content will be measured with MRS. Whole-body oxidation of lipids, carbohydrates and protein will be determined using indirect calorimetry.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
10
Ketogenic dietary intervention
Simple calorie restriction dietary intervention
Biomedicum 2U
Helsinki, Uusimaa, Finland
Change in hepatic triglyceride content
Change in hepatic triglyceride content will be measured using 1H magnetic resonance spectroscopy (MRS).
Time frame: 1 week
Change in hepatic mitochondrial flux
Change in the rate of hepatic mitochondrial flux (Vcs) will be assessed using 3-13C-lactate infusion.
Time frame: 1 week
Change in beta-hydroxybutyrate production rate
Change in the rate of beta-hydroxybutyrate production will be assessed using \[13C4\]β-hydroxybutyrate infusion.
Time frame: 1 week
Change in glucose production rate
Change in the rate of glucose production will be assessed using \[2H7\]glucose infusion.
Time frame: 1 week
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