The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, the normal variation of various bioactive compounds and their levels is required to be able to develop formulas better corresponding the breast milk, and ensure the optimal development of also the formula-fed babies.
Study Type
OBSERVATIONAL
Enrollment
15
A single breast-milk sample will be collected from 20 women. Milk samples will be pooled for analysis.
Department of Biochemistry, University of Turku
Turku, Finland
RECRUITINGRelative proportions (%) of regioisomers within selected molecular species (acyl carbon number:number of double bonds 50:1 and 52:2) in triacylglycerols.
Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Negative ionization of the triacylglycerols with ammonia. Selection of the molecular weight species in the first quadruple of the MS/MS. Fragmentation of the selected ions with argon. Separation of the fragment ions in the second quadrupole of the MS/MS. Calculation of the relative proportions of regioisomers with a computer software.
Time frame: 11/2018-12/2019
Relative proportions (%) of fatty acids in triacylglycerols of human milk.
Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Quantitation with gas chromatograph coupled with flame ionization detector in relation to external standards.
Time frame: 11/2018-12/2019
Quantity (mg/ml) of lipid soluble compounds in triacylglycerols of human milk.
Isolation of lipids from the milk with solvent extraction. Gravimetric analysis of the lipids.
Time frame: 11/2018-12/2019
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.