Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization

Swiss Federal Institute of Technology18 enrolled

Overview

It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated. The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation. The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Healthy

Interventions

Eligibility

Sex: MALEMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Non-smoking * Healthy * Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2 * Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years) * Willing to adhere to the study rules Exclusion Criteria: * Known or suspected non-compliance, drug or alcohol abuse * Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant * Previous enrolment into the current study * Enrolment of the investigator, his/her family members, employees and other dependent persons * Regular intake of medication * Regular intake of antioxidant supplementations

Outcomes

Primary Outcomes

Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation

Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.

Time frame: On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)

Secondary Outcomes

Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation

Numbers of apoptotic mononuclear cells, apoptotic hematopoietic, endothelial and mesenchymal stem and progenitor cells and apoptotic mature endothelial cells, as well as in oxidative stress markers in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.