Intestinal Microbiota, Tryptophan and Autism

N/ACompletedNCT03751930

University Hospital, Tours66 enrolled

Overview

Autism Spectrum Disorder refers to complex neuro-developmental disorders that affect social communication and behavioral adaptation. Currently, the diagnosis of Autism Spectrum Disorder is based on a clinical examination that is performed classically during the first three years of life. The heterogeneity of the disorders occurring in autism make pathologies difficult to diagnose and manage. The overall goal of this project is the identification of metabolic biomarkers based on clinical profile. The best characterization of physiopathological pathways will ultimately allow the identification of subgroups of subjects and facilitate the development of targeted therapeutics. The proposed work aims to test the hypothesis of a disruption of tryptophan metabolism in Autism Spectrum Disorder via the gut microbiota.

Autism Spectrum Disorder refers to complex neuro-developmental disorders that affect social communication and behavioral adaptation. In France, Autism Spectrum Disorderaffects about 1 in 100 people according to international criteria and is diagnosed in early childhood. Currently, the diagnosis of Autism Spectrum Disorder is based on a clinical examination that is performed classically during the first three years of life. The heterogeneity of the disorders occurring in autism make pathologies difficult to diagnose and manage. The overall goal of this project is the identification of metabolic biomarkers based on clinical profile (based on behavioral and cognitive markers). The best characterization of physiopathological pathways (from the molecular scale to the phenotypic scale) will ultimately allow the identification of subgroups of subjects and facilitate the development of targeted therapeutics. The proposed work aims to test the hypothesis of a disruption of tryptophan metabolism in Autism Spectrum Disorder via the gut microbiota.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Autism Spectrum Disorder

Eligibility

Sex: ALLMin age: 3 YearsMax age: 12 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria (patient): * 3-12 year old child * Diagnosis of Autism Spectrum Disorders according to the DSM-5 (2013), invasive developmental disorder according to the ICD-10 (1993) or Autism Spectrum Disorders according to ICD-11 (2018) * Affiliate or beneficiary of a social security scheme * Consent signed by at least one of the parents Exclusion Criteria (patient): * Chronic inflammatory pathology * Probiotic intake * Taking medication (except melatonin) within 6 days before inclusion and until biological samples are collected Inclusion Criteria (healthy volunteer): * 3-12 year old child * Affiliate or beneficiary of a social security scheme * Consent signed by both parents Exclusion Criteria (healthy volunteer): * Chronic inflammatory pathology * Digestive pathology * Probiotic intake * Taking medication within 6 days before inclusion and until biological samples are collected * Personal and / or family history of neurological disease, epilepsy, psychiatric disorder, neurodevelopmental disorder, language disorder, overdrive, intellectual disability

Outcomes

Primary Outcomes

Urinary concentrations of tryptophan metabolites

Quantification by High-Performance Liquid Chromatography and Mass Spectrometry

Time frame: At baseline

Faecal concentrations of tryptophan metabolites

Quantification by using High-Performance Liquid Chromatography and Mass Spectrometry

Time frame: At baseline

Secondary Outcomes

Characterization of gut microbiota

Characterization by 16S ribosomal RNA sequencing

Time frame: At baseline

Correlation of metabolic profiles of tryptophan and microbiotic profiles

Study of correlations from the outcomes measures 1, 2 and 3

Time frame: At baseline

Intestinal Microbiota, Tryptophan and Autism

Overview

Conditions

Interventions

Eligibility

Locations (2)

Outcomes

Primary Outcomes

Secondary Outcomes