To elucidate the longitudinal development of intestinal microbiota in patients with IL10RA deficiency after hematopoietic stem cell transplantation (HSCT). The investigators planned to collect fecal samples from IL10RA-deficient patients who received HSCT. Samples were collected more than once every three days after engraftment in lamina flow ward and at least once a week before discharge. Microbial DNA was extracted from the fecal samples. And all analysis was based on the next generation sequencing data.
Hematopoietic stem cell transplantation is considered the only curative therapy for patients with interleukin-10 receptor-A(IL10RA) deficiency. The investigators aimed to collect the fecal samples of these patients throughout the conditioning, transplantation until discharge, providing a dense insight into the longitudinal development of intestinal microbiota.
Study Type
OBSERVATIONAL
Enrollment
13
1. Reduced intensity conditioning(RIC) chemotherapy regimen before transplantation 2. All patients were cared for in single rooms ventilated with a highly effective particulate air filtration system. All patients received intravenous immunoglobulin and antimicrobial prophylaxis, which included antiviral, antifungal, and Pneumocystis jirovecii prophylaxis with ganciclovir and micafungin, as per routine clinical practice before transplantation. 3. Umbilical cord blood transplantation
Children's hospital of Fudan university
Shanghai, Shanghai Municipality, China
Shannon indices
Shannon index is a popular diversity index in the ecological literature; minimum score is zero, higher values represent a better outcome
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
Simpson indices
Simpson index in ecology is to measure the degree of concentration when individuals are classified into types; scale ranges \[0,1\]; lower values represent a better outcome
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
relative abundance of Proteobacteria
describe the microbiome at the phylum level
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
relative abundance of Firmicutes
describe the microbiome at the phylum level
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
Clostridium sensus_stricto_1
describe the microbiome at the genus level
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
Escherichia - Shigella
describe the microbiome at the genus level
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
chimerism
The level of chimerism of each patient will be reported as percentages \[0,100%\]
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Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
manifestation of graft-versus-host disease
describe the graft-versus-host disease happened to each patient
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
Sobs indices
describe the the observed richness of each sample
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
Chao1 estimator
describe the operational taxonomic unit(OTU) numbers of each sample
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
neutrophil cell count
blood test results
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient
platelet count
blood test results
Time frame: during the hospitalization of each eligible patient, an average of 90 days per patient