Objective: The aim of this study is to evaluate the effect of HA as an adjunct to scaling and root planning (SRP) on clinical parameters, periodontal inflamed surface area (PISA) and adenosine deaminase (ADA), catalase (CAT), and glutathione (GSH) levels in gingival crevicular fluid (GCF) in patients with periodontitis.
Design: A total of 24 Stage II-III periodontitis patients will be included in this split-mouth, randomized-controlled study. The study population will be divided into four groups: in Group 1 (SRP): SRP+ saline; in Group 2 (SRP+HAgel): SRP + HA gel; in Group 3 (SRP+HAmo): SRP+ HA mouthrinse; and in Group 4 (SRP+HAmo+HAgel): SRP+HA mouthrinse + HA gingival gel were applied. At baseline and Week 4 periodontal clinical parameters and PISA will be calculated. Also, GCF amount will be collected to evaluate ADA, CAT, and GSH by spectrophotometric analysis.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
TRIPLE
Enrollment
24
Under local anesthesia, full-mouth SRP will be performed by a single investigator. Immediately after the SRP, HA gel or mouthrinse will perform according to the groups' procedure. In Group 1, the periodontal sulcus will be irrigated with saline solution after SRP.
In Group 2, after SRP will be performed and irrigated with saline the area was dried with a soft air and, then, a gel containing HA will be applied intrasulcular.
In Group 3, HA hydrogel mouthrinse will be used as an irrigator after SRP.
Van Yuzuncu Yil University Faculty of Dentistry
Van, Turkey (Türkiye)
plaque index
in accordance with the Sillness and Löe index (Silness \& Löe, 1964)
Time frame: Change from Baseline to week 4
gingival index (GI)
confirming to Silness and Löe index (Löe \& Silness, 1963)
Time frame: Change from Baseline to week 4
bleeding on probing (BOP)
according to Ainoma and Bay (AINAMO \& Bay, 1975);
Time frame: Change from Baseline to week 4
Probing depth (PD)
as the measurement of the length between the bottom of the periodontal pocket to marginal gingiva;
Time frame: Change from Baseline to week 4
gingival recession height (GRH)
as the measurement of the distance between the marginal gingiva and cemento-enamel junction
Time frame: Change from Baseline to week 4
clinical attachment level (CAL)
as the sum of gingival recession height and probing depth will be recorded.
Time frame: Change from Baseline to week 4
Changes of Periodontal inflammed surface area
All clinical parameters will be entered on Microsoft Excel Program (www.parsprototo.info) which constructed for calculating PISA. The BOP percentage for each tooth will be calculated by entering "number sites with BOP" values in the Excel program.
Time frame: Change from Baseline Baseline to week 4
Changes of Adenosine deaminase (ADA) levels in gingival crevicular fluid
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
In Group 4, after SRP will be performed, the sulcus was irrigated with HA hydrogel mouthrinse and, then, intrasulculary HA gingival gel will be applied.
The ADA activity will be evaluated spectrophotometrically
Time frame: Changes from Baseline to week 1,2,4
Changes of Catalase levels in gingival crevicular fluid
The catalase activity will be evaluated spectrophotometrically
Time frame: Changes from Baseline to week 1,2,4
Changes of Glutathione levels in gingival crevicular fluid
The Glutathione activity will be evaluated spectrophotometrically
Time frame: Changes from Baseline to week 1,2,4