Tenofovir/Levonorgestrel Intravaginal Ring and Tenofovir Intravaginal Ring

Inclusion Criteria: * Female, aged 18-34 years, inclusive * General good health (by history and per clinician discretion) without any clinically significant systemic disease (including, but not limited to significant liver disease/hepatitis, gastrointestinal disease, kidney disease, thyroid disease, osteoporosis or bone disease, and diabetes), uterus, and cervix * Not pregnant or planning to become pregnant * Pre-screening HIV risk score ≤4 * Currently having regular menstrual cycles (approximately 24-35 days) OR with a history of having regular menstrual cycles before contraceptive use, by report, and resumed some menstruation or spotting (with biochemical confirmation of ovulation) * Willing to undergo Visual Inspection with Lugol's Iodine (VILI) for cervical abnormalities during pelvic exam * Willing to abstain from use of vaginal products other than the study product, including tampons (except for during menses) , menstrual cups, vaginally inserted cloths or other materials, spermicides, lubricants, and douches for the whole study * Willing to abstain from any vaginal intercourse starting 48 hours before certain study visits * Vaginal and cervical anatomy that, in the opinion of the clinician, lends itself to easy genital tract sample collection and is absent of vesicles and ulcers * No use of hormonal contraceptives within the following periods specified for each type of contraception method: * Oral contraceptives (combined or progestin-only), contraceptive patch or contraceptive vaginal ring in at least two (2) months * Last DMPA injection received at least four (4) months ago and has resumed regular menstruation * Hormonal IUD/IUS removed at least four (4) months ago and has resumed regular menstruation * Hormonal implant removed at least six (6) months ago and has resumed regular menstruation * Willing to refrain from using any hormonal contraceptives for the entire study and to use only study-provided non-spermicidal male condoms with or without a study-provided Cu-IUD * P4 ≥3.0 ng/ml * Estimated glomerular filtration rate (eGFR) ≥90ml/min/1.73m2 * Willing to give voluntary consent and sign/mark an informed consent form * Willing and able to comply with protocol requirements Exclusion Criteria: * Body mass index (BMI) ≥30 kg/m * History of hysterectomy * Currently pregnant or within less than three (3) calendar months of the last pregnancy outcome. * Currently breastfeeding or having breastfed an infant in the last two (2) months, or planning to breastfeed during the course of the study * Contraindication to any study products-LNG, TFV, or excipient ingredients * Contraindication to LNG * In the last three (3) months, diagnosed with or treated for any STI or pelvic inflammatory disease * Positive test for HIV-1, syphilis, Trichomonas vaginalis (TV), Neisseria gonorrhea (GC), Chlamydia trachomatis (CT) or HBsAg * Nugent score greater than or equal to 7 or a symptomatic BV clinical diagnosis as defined by Amsel's criteria * Suspected breast cancer or other progestin-sensitive cancer * Suspected hepatic disease, including cirrhosis or viral hepatitis * History of bleeding or coagulation problems * Known current drug or alcohol abuse which could impact study compliance * Grade 2 or higher laboratory abnormality, per the Division of AIDS, National Institute of Allergy and Infectious Disease (DAIDS) Table for Grading the Severity of Adverse Events, or clinically significant laboratory abnormality as determined by the clinician * Use of any concomitant medications * Participation in any other investigational trial with use of a drug/device within the last 30 days or planned participation in any other investigational trial with use of a drug/device during the study * History of gynecological procedures (including genital piercing) on the external genitalia, vagina, or cervix within the last 14 days * Labial elongation (due to pulling practices and use of botanicals or caustic agents) * Abnormal finding on laboratory or physical examination or a social or medical condition in the volunteer which, in the opinion of the site co-PI(s), would make participation in the study unsafe or would complicate interpretation of data * Currently using, or has used within the preceding one (1) month, emtricitabine/tenofovir disoproxil fumarate (TDF/FTC or Truvada®) or any other tenofovir product, and/or has plans to use a non-study tenofovir product in any form during the study