This project is expected to confirm the hypothesis that hyposplenism in patients with celiac disease is not conditioned by a selective memory deficiency of B lymphocytes. Other objectives of project are: * determination of gliadin 33-mer in faecal and urine as indicators patient´s adherence to gluten-free diet * determination of citrulline in plasma as an indicator of the overall functional capacity enterocytes
Ultrasound examination is focused to spleen size (spleen volumetry). Methodology of laboratory examinations is based on venous blood sampling for the determination of memory B-like marginal zone by flow cytometry; gliadin 33-mer (ELISA) determination in faecal and urine sample; blood plasma extraction for citrulline (high performance liquid chromatography, HPLC).
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
50
Venous blood samples to determine memory B lymphocytes.
Ultrasound examination of spleen to scaled the size.
University Hospital Hradec Králové
Hradec Králové, The Czech Republic, Czechia
RECRUITINGconcentration of B-lymphocytes as parameter to evaluate treatment effect
assessment method: blood sampling, units: the measurement results are in absolute numbers, method: flow cytometry
Time frame: one year
concentration of B-lymphocytes as parameter to evaluate treatment effect
assessment method: blood sampling, units: the measurement results are in percentage numbers, method: flow cytometry
Time frame: one year
concentration of citrullin in plasma as parameter to evaluate treatment effect
assessment method: blood sampling, units: μmol/l (micromol/l; Citrullin cut-off for intestinal failure is \<20μmol / L), method: mass spectrometry (LC-MS instrumentation with internal isotopically labeled standard)
Time frame: one year
concentration of ornithin in plasma as parameter to evaluate treatment effect
assessment method: blood sampling, units: μmol/l (micromol/l), method: mass spectrometry (LC-MS instrumentation with internal isotopically labeled standard)
Time frame: one year
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