The primary objective of this study is to determine the effects of different methods of broccoli preparation on levels of urinary glucosinolate metabolites. The secondary objective is to determine the effects of different methods of broccoli preparation on levels of plasma carotenoids.
Consumption of Brassica vegetables (which include broccoli, cabbage, and kale) is inversely associated with the incidence of several cancers, including cancers of the lung, stomach, liver, colon, rectum, breast, endometrium, and ovaries. Brassica vegetables are a good source of many nutrients including glucosinolates and carotenoids. Glucosinolates are sulfur-containing compounds that are converted to bioactive metabolites by an enzyme called myrosinase, which is released when the vesicles containing myrosinase are ruptured by chewing or cutting. These bioactive compounds are considered to be active agents for cancer prevention. Their ability to reduce risk of cancer may derive in part from their ability to modulate foreign-substance metabolizing enzymes, which include enzymes called Phase I cytochrome P450s and Phase II enzymes. Carotenoids may also have a role in cancer prevention and other health benefits including reducing age-related macular degeneration, and therefore merit research to reveal factors that affect their presence in the body. The primary aim of this study is to investigate how various preparations of broccoli impact levels of these healthful nutrients in humans.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
DOUBLE
Enrollment
16
Combination of Chips, Pretzels, and Popcorn.
Oven-roasted, freeze-dried, cheese flavored broccoli
Oven-roasted, freeze-dried, cheese flavored broccoli with Daikon radish powder
USDA-ARS Beltsville Human Nutrition Research Center
Beltsville, Maryland, United States
Glucosinolate Metabolites will be measured
Urine will be analyzed for glucosinolate metabolites
Time frame: Day 1
Glucosinolate Metabolites will be measured
Urine will be analyzed for glucosinolate metabolites
Time frame: Day 22
Glucosinolate Metabolites will be measured
Urine will be analyzed for glucosinolate metabolites
Time frame: Day 43
Glucosinolate Metabolites will be measured
Urine will be analyzed for glucosinolate metabolites
Time frame: Day 64
Carotenoids will be measured
Carotenoids will be extracted from plasma
Time frame: Days 1, 2, 3, 4, 11, 22, 23, 24, 25, 32, 43, 44, 45, 46, 53, 64, 65, 66, 67, 74
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Uncooked, freeze-dried broccoli with ranch-type dip