Characterization of Gut Microbiota Composition and Activity After a Daily Supplementation of 4.5 g/Day of ChitinGlucan Fibre During 3 Weeks in At-cardiometabolic Risk Volunteers

gut microbiota composition

Change from baseline gut microbiota composition by 16SrDNA Illimina Sequencing

Time frame: baseline and three weeks

fecal biliary acids

Change from baseline fecal biliary acids by FAME quantification (gas-liquid chromatography)

Time frame: baseline and three weeks

fecal PolyUnsaturated Fatty Acids (PUFA)

Change from baseline fecal PolyUnsaturted Fatty Acids (PUFA) by FAME quantification (gas-liquid chromatography)

Time frame: baseline and three weeks

fecal albumin

Change from baseline fecal albumin by immunoenzymatic technique (ELISA)

Time frame: baseline and three weeks

fecal zonulin

Change from baseline fecal zonulin by immunoenzymatic technique (IDK Zonulin ELISA)

Time frame: baseline and three weeks

fecal calprotectin

Change from baseline fecal calprotectin by Phadia 100 system

Time frame: baseline and three weeks

TLR-agonist in stool

Change from baseline TRL-agonist in stool using the HEK-Blue™ TLR cellules

Time frame: baseline and three weeks

Change from baseline CO2, CH4 and H2 kinetics in exhaled gases

by gas chromatography, during ten hours after rich-fiber breakfast and nutritional challenge test at lunch

Time frame: baseline and three weeks

body composition

Change from baseline bodycompositio by bioimpedancemetry

Time frame: baseline and three weeks

Change from plasma glucose kinetics

by spectrophotometry method , during seven hours after rich-fiber breakfast and nutritional challenge test at lunch

Time frame: baseline and three weeks

Change from plasma insulin kinetics

by radio-immuno analysis , during seven hours after rich-fiber breakfast and nutritional challenge test at lunch

Time frame: baseline and three weeks

Change from plasma Non-Esterifies Fatty-Acid (NEFA) kinetics

by spectrophotometry method , during seven hours after rich-fiber breakfast and nutritional challenge test at lunch

Time frame: baseline and three weeks

plasma TriacylGlycerol (TG) kinetics

Change from plasma TriacylGlycerol (TG) kinetics by spectrophotometry method , during seven hours after rich-fiber breakfast and nutritional challenge test at lunch

Time frame: baseline and three weeks

Cholesterol Total, Cholesterol LDL

Change from baseline cholesterol Total, Cholesterol LDL by spectrophotometry method

Time frame: baseline and three weeks

resting energy expenditure

Change from baseline resting energy expenditure by indirect calorimetry

Time frame: baseline and three weeks

stool consistency

by Bristol Stool Chart (type1-7)

Time frame: every week, up to three weeks

stool frequency

by questionnaire

Time frame: every week, up to three weeks

gastro-intestinal symptoms

by questionnaires and visual analogue scale (VAS) score (on a 90mm horizontal line; from no symptom (minimal) to serious symptom (maximum))

Time frame: every week, up to three weeks

quality of life during the study

change from baseline quality of life during the study by SF 36 questionnaire

Time frame: baseline and three weeks

change from baseline dietary intake of fibre

by three day food record

Time frame: baseline and three weeks