The pH stability during human gamete handling for intracytoplasmic sperm injection (ICSI) is physiologically sound. As the pH change for bicarbonate-buffered medium, scientists assumed whether the use of HEPES or MOPS buffered medium would provide more stable pH, thereby better outcomes. However, the oil use and time frame outside the incubators have not been accounted for. This study is to evaluate the use of HEPES buffered medium compared with the bicarbonate-buffered medium on ICSI outcomes.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
TRIPLE
Enrollment
1,057
The investigators will evaluate whether the usual bicarbonate culture medium can replace the HEPES buffered medium effectively.
Qena Fertility Center
Qina, Qena Governorate, Egypt
Banon Assiut
Asyut, Egypt
IbnSina IVF Center
Sohag, Egypt
live birth rate
viable neonate after 30 weeks of Gestation
Time frame: 40 weeks
Clinical pregnancy rate
a positive heartbeat on ultrasound at ≥ 7 weeks of gestation
Time frame: 12 weeks
biochemical pregnancy rate
positive b-hCG at ≥ 14 days following embryo transfer
Time frame: two weeks
embryo developmental rates
fertilized oocyte per injected ones and developed embryos per fertilized oocytes
Time frame: 6 days of culture
Ongoing pregnancy rate
Continued pregnancy after week 12 of gestation
Time frame: 16 weeks
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