Effect of Packed Red Blood Cell Transfusion on Eicosanoid Profiles in Plasma of Intensive Care Patients

Medical University of Vienna23 enrolled

Overview

The main goal of this pilot study is to assess the time course of eicosanoid profiles in intensive care unit (ICU) patients requiring packed red blood cell (PRBC) transfusion. Moreover we will analyze the change of levels of eicosanoids in patient plasma prior and after a PRBC transfusion as well as its correlation with levels of eicosanoids in the transfused PRBCs. These data will then be used to determine the estimated effect size necessary for the planning of future larger studies. We hypothesize that transfusion of PRBCs will modulate the eicosanoid profile in ICU patients. According to the Protocol filed with the Institutional Review Board of the Medical University of Vienna and patient's informed consent, subsequent sub analyses using samples of this study (e.g., determination of extracellular vesicles in PRBC samples and patient's plasma) will be performed.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Anemia Critical Illness Lung Transplantation Open Aortic Surgery

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 99 Years

Medical Language ↔ Plain English

Exclusion Criteria: * Age \<18 years or \>99 years * Pregnancy * \<12h since last PRBC transfusion * intake/administration of acetylsalicylic acid in groups 2 and 3 * intake/administration of cysteinyl leukotriene receptor antagonists (e.g. montelukast) * intake/administration of celecoxib, etoricoxib, parecoxib, ibuprofen, diclofenac, and naproxen in all groups * glucocorticoids given within 24h of transfusion in groups 1 and 3 * treatment with calcineurin inhibitors (e.g. tacrolimus) in groups 1 and 3 * treatment with alemtuzumab in groups 1 and 3 * administration of protamine for heparin reversal in groups 2 and 3

Outcomes

Primary Outcomes

Change of plasma eicosanoid concentrations in ICU patients subjected to PRBC Transfusion at any time Point compared to baseline

Time frame: 60 minutes prior to 90 minutes after packed red blood cell transfusion

Secondary Outcomes

Changes of plasma eicosanoid profiles after PRBC transfusion compared to prior to PRBC transfusion in ICU patients

Time frame: 60 minutes prior to 90 minutes after packed red blood cell transfusion

Correlation of plasma eicosanoid profiles in the transfused PRBCs and the change of their plasma levels in ICU patients

Time frame: 60 minutes prior to 90 minutes after packed red blood cell transfusion

Effect of Packed Red Blood Cell Transfusion on Eicosanoid Profiles in Plasma of Intensive Care Patients

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes