Investigation of Microbiome-Derived Metabolite Production in Response to a Mediterranean Diet

Laval University21 enrolled

Overview

This project proposes to characterise the microbiome-derived metabolites produced upon ingestion of bioactive precursor-rich meals by healthy volunteers in order to investigate the relationship between gut microbiome composition and the nature of dietary metabolites production.

There is a large inter-individual variability in gut microbiome-derived metabolites production upon ingestion of similar meals. Since many of these microbiome-derived metabolites have enteric and systemic biological effects, these variabilities could in part account for the different responses to meals and diets. This project thus aims to improve the understanding the relationship between gut microbiome composition and the nature of dietary metabolites produced from similar bioactive precursor-rich meals. The investigators therefore propose to characterise the circulating microbiome-derived metabolite profiles in healthy individuals fed the same 2-day precursor-rich Mediterranean diet prior to and after a 2-week of habitual Canadian diet feeding.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Food Habits

Interventions

Mediterranean dietOTHER

During both Mediterranean diet periods, participants will be provided with meals and snacks representative of the Mediterranean nutritional habits. Participants will be required to eat only the meals provided.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Men and women aged between 18 and 35 years * Body mass index between 18.5 and 30 kg/m2 * General good health * Computer and Internet access * Basic understanding of written French Exclusion Criteria: * Intestinal pathologies including inflammatory bowel disease and gastrointestinal cancers * Pregnant and/or breast-feeding women * Alcohol consumption greater than 15 portions for men and 10 for women weekly * Active tobacco usage * Consumption of dietary supplements (e.g. multivitamins, omega-3, probiotics) * Completion of a course of antibiotics in the past 3 months * Regular use of anti-inflammatory drugs, or use in the past 3 months * Important weight change (+/- 5 kg) in the past 6 months * Allergies or food intolerances to ingredients present in trial diets

Locations (1)

Institut sur la nutrition et les aliments fonctionnels - INAF

Québec, Quebec, Canada

Outcomes

Primary Outcomes

Microbiome-derived metabolite profiling

Plasma and urine metabolomics profiling of gut-microbiome derived metabolites (e.g., branched-chain amino acids \[BCAA\], short chain fatty acids \[SCFA\], trimethylamine \[TMA\], amino acids- and polyphenol-metabolites) will also performed at the same time points.

Time frame: 19 days

Gut microbiome characterisation

Fecal microbiome composition will be characterise by rRNA 16S sequencing prior to and following both 2-day Mediterranean diet periods. Relevant indexes of microbiome diversity (e.g. Shannon beta-diversity), ratios between meaningful bacterial markers of gut dysbiosis (e.g. Firmicutes:Bacteroidetes) and specific microbiome differences in bacterial Families, Genera or species between study periods and individuals.

Time frame: 19 days

Secondary Outcomes

Endocannabinoid interaction with interindividual microbiome composition

Interindividual variations in the endocannabinoid levels following a 2-day Mediterranean diet intervention according to individual gut microbiome composition will also be characterised. N-acyl ethanolamines, acylglycerols and omega-3 derived endocannabinoids will be quantified by LC MS-MS in plasma and urine samples collected prior to and following both 2-day Mediterranean diet periods.

Time frame: 19 days

Data from ClinicalTrials.gov

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