Reversal of Dual Antiplatelet Therapy With Cold Stored Platelets

Inclusion Criteria: The subject is in good health, is taking no excluded medications and meets platelet donor suitability requirements aimed at assuring donor safety. 1. Subject self-reports that he or she feels well and healthy 2. Subjects must be 18-59 years old, of either sex 3. Temperature: less than or equal to 99.5 F 4. Resting blood pressure: systolic less than or equal to 180 mmHg, diastolic less than or equal to100 mmHg 5. Resting heart rate: 40 to 100 beats per minute 6. Weight: greater than or equal to110 pounds, 7. Hematocrit: greater than or equal to 35 percent for females, greater than or equal to 38 percent for males, but not greater than 55 percent 8. Platelet count able to achieve target platelet yield per apheresis machine configuration parameters 9. Subjects must be able to read, understand and sign the informed consent document and commit to the study follow-up schedule. The ability to read and speak English is required for participation. 10. Subject must agree to avoid taking any aspirin or aspirin-containing drugs (e.g., Alka-Seltzer, Bufferin, Excedrin) or NSAIDs (e.g.,Feldene, Motrin, Aleve, Advil) or other drugs known to affect platelet function throughout their study participation. 11. Subjects must agree to avoid calcium channel blockers such as amlodipine (Norvasc), felodipine, and verapamil (Verelan, Calan) and proton pump inhibitors (PPIs) such as omeprazole (Prilosec), lansoprazole (Prevacid), esomeprazole (Nexium) and pantoprazole (Protonix) until all study blood draws are concluded. These classes of drugs may interfere with the action of clopidogrel and diminish its antiplatelet effect. 12. Subjects must have "good veins" for apheresis platelet collection and drawing blood samples. 13. Women of child bearing potential must agree to use an effective method of contraception during the course of the study. The following methods of contraception will be considered 'effective' when self-reported by subject; abstinence, intrauterine contraception devices, hormonal methods, barrier methods or history of sterilization. 14. Subject has phone and e-mail for contact and notification, and is able to come to the research site for approximately 9 visits over up to approximately 64 days. Exclusion Criteria: Healthy subjects will be excluded from the study for any of the following reasons: 1. Active acute infection or suspected active infection, temperature above 100 F or taking antibiotic 2. Active immune/inflammatory condition (e.g. gout, systemic lupus erythematosus, allograft rejection) 3. History of heart disease, including endorsement of shortness of breath with mild exertion (at the discretion of the PI). 4. History of significant liver, kidney, GI, blood, endocrine/metabolic, autoimmune or pulmonary disease, untreated hypertension and or metabolic syndrome (at the discretion of the PI). 5. Diabetes Mellitus 6. Cancer of any kind (exceptions being basal or squamous cell cancers of the skin), under treatment or resolved 7. History of bleeding events, family history of bleeding events, or known genetic disorder with bleeding diathesis 8. A family history of venous or arterial thrombosis before the age of 50 in first degree relatives 9. A personal history of DVT, venous or arterial thrombosis, blood clots or stroke 10. History of having been prescribed clopidogrel (Plavix), ticlopidine (Ticlid) or Ticagrelor (Brilinta). 11. Subject has taken any aspirin or aspirin-containing drugs (e.g., Alka-Seltzer, Bufferin, Excedrin) or NSAIDs (e.g.,Feldene, Motrin, Aleve, Advil) or other drugs known to affect platelet function within 14 days prior to screening. 12. Chronic NSAID therapy 13. Chronic steroid therapy 14. Known allergy to aspirin or clopidogrel 15. Subjects who are taking, or have taken within 14 days any of the following class of drugs protone pump inhibitors (PPIs) such as omeprazole, pantoprazole, lansoprazole, esomeprazole lipid lowering drugs/statins anticoagulants ACE-inhibitors phenytoine tolbutamide calcium channel blockers such as amlodipine, felodipine, verapamil 16. Current drug or alcohol dependence by subject's declaration. 17. Currently pregnant or nursing as assessed during interview. A urine pregnancy test prior to apheresis is required for women of childbearing potential. 18. Subject plans to participate in contact sports during study/observational period such as boxing, rugby, American football, soccer, or other risky recreational hobbies at the discretion of the investigator. 19. Unwilling or unable to comply with the protocol in the opinion of the investigator. 20. Participation in an experimental drug/device study within the past 30 days (other than this study). Subjects who have received an infusion on this study may not be re-enrolled. 21. Average initial (only for the first PFT) aggregation responses to ADP less than or equal to 60% by aggregometry platelet function testing. 22. Other unspecified reasons that, in the opinion of the Investigator, make the subject unsuitable for enrollment.