PIRAMIDE study design will test the hypothesis that simultaneous interactions between DNA methylation and microRNAs may hit T2D candidate genes and predict the development of T2D-related cardiovascular complications.
Study Type
OBSERVATIONAL
Enrollment
35
Università della Campania Luigi Vanvitelli
Naples, Italy
RECRUITINGDNA methylation status both of CD4+ and CD8+ cells
Using Methylation 27K BeadChip platform based on Bisulfite conversion technology, DNA methylation profile of 35 preselected T2D patients will be measured both in CD4+ and CD8+ cells collected from peripheral blood respect with controls.
Time frame: 3 Months
Bioinformatics analysis to predict putative novel T2D candidate genes
The network-based algorithm "Weighted Human DNA methylation PPI network (WMPN)" will be applied to methylome data in order to obtain a disease module containing the crucial differentially methylated genes both in CD4+ and CD8+ cells from T2D patients and controls.
Time frame: 6 Months
RNA sequencing analysis both in CD4+ and CD8+ cells
RNA sequencing analysis by using Illumina HiSeq2000 Next Generatin Sequencing (NGS) platform will be performed for identifying differentially expressed micro-RNA and mRNA target both in CD4+ and CD8+ cells isolated from T2D patients and controls .
Time frame: 3 Months
ROC curves to evaluate putative DNA methylation/microRNA interactions as prognostic biomarkers of cardiovascular dysfunction in T2D patients
The ROC curves will be used to correlation putative epigenetics interactions (DNA methylation and microRNA) with the presence or absence of T2D-related cardiovascular dysfunction.
Time frame: 12 Months
Claudio Napoli, Professor
CONTACT
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