Pediatric Obesity Related Metabolite Profile and Gut Microbiota

Hallym University Kangnam Sacred Heart Hospital60 enrolled

Overview

In this study, the investigators aimed to observe changes in the gut microbial composition before and after the weight-loss intervention, including dietary control and exercise, in obese children. The main metabolites produced by gut microbiome, short chain fatty acids (SCFAs) will be also analysed. The investigators will analyse the change of gut microbiome composition and metabolites related with weight-loss intervention and the relationship between microbiome composition and metabolites.

Many recent studies have suggested that dysbiosis of the gut microbiome might related to obesity. There is growing evidence that the composition of gut microbiome changes in childhood obesity compared to normal-weight children, and it is attracting attention as an adjustable environmental factor in obesity treatment. It has been reported that gut microbiome could be changed due to environmental influences such as diet and exercise. In this study, the investigators aimed to observe changes in the gut microbial composition before and after the weight-loss intervention, including dietary control and exercise, in obese children. The main metabolites produced by gut microbiome, short chain fatty acids (SCFAs) would be also analysed to investigate the relationship with gut microbiome and with weight-loss intervention. Intervention is not performed for normal-weight children, and samples from normal-weight children will be used as a control for gut microbiome and biochemical tests.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

SUPPORTIVE_CARE

Masking

NONE

Enrollment

Conditions

Obesity, Childhood

Interventions

Individualized Education ProgramBEHAVIORAL

The every participants will have individualized education program about a way of dietary control and exercise in their usual life.

Eligibility

Sex: ALLMax age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Obese children: Children ≥95 ‰ * Non-obese children: 5‰\<BMI \<85 ‰ Exclusion Criteria: * Taking antibiotics, probiotics, or steroids for a month before visit * Taking probiotics-like products including yogurt for seven days before visit * Having enteritis symptoms including diarrhea for a month before visit * Chronic heart disease, chronic bowel disease, chronic liver disease, chronic kidney disease, endocrine disease, genetic diseases or congenital metabolic disorder

Locations (1)

Hallym University Kangnam Sacred Heart Hospital

Seoul, South Korea

Outcomes

Primary Outcomes

Change in gut microbiome composition in stool samples

Bacterial diversity and composition using 16s rRNA sequencing. Especially Bacteroidetes and Firmicutes populations in fecal microbiome will be analyzed related with obesity.

Time frame: up to 3 months after enrollment

Secondary Outcomes

Changes in short chain fatty acids of fecal samples

The main metabolites produced by gut microbiome, short chain fatty acids using gas chromatography- mass-spectrometry.

Time frame: up to 12 months after enrollment

Data from ClinicalTrials.gov

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