Effect of Mango on Gut Microbiota and Metabolic Health

Laval University35 enrolled

Overview

Mango is rich in bioactive compounds such as dietary polyphenols and carotenoids, which may explain its beneficial effect on health. Polyphenols accumulate in the gut where they can positively modulate the microbiota. As gut microbiota may have a strong influence on cardiometabolic health, we hypothesize that mango consumption improves metabolic profile in overweight or obese individuals through beneficial changes in gut microbiota. The study of metagenomics, transcriptomics and metabolomics will be used to validate this hypothesis.

Study Type

INTERVENTIONAL

Allocation

Purpose

PREVENTION

Masking

NONE

Enrollment

Conditions

Gut Microbiota Metabolic Syndrome

Interventions

Mango consumptionDIETARY_SUPPLEMENT

Daily consumption of 280 g of frozen mangos during 8 weeks. The daily dose in equivalent to a 2-cups serving.

Eligibility

Sex: ALLMin age: 18 YearsMax age: 55 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy men and premenopausal women * At least one of the following : BMI between 25 and 40 kg/m2 or Waist circumference ≥ 80 cm for women and ≥ 94 cm for men * At least one of the following : TG ≥ 1.35 mmol/L or fasting insulinemia ≥ 42 pmol/L * Caucasian origin Exclusion Criteria: * Nicotine use * Metabolic disorders or use of medication that may affect parameters measured in the study * Use of dietary supplements or natural health product that may affect parameters measured in the study * Use of antibiotics in the last 3 months * Surgery in the last 3 months or planned during the study timeline * Alcohol consumption \>2 drinks/day * Weight change \>5% in the last 3 months * Mango aversion, allergy or intolerance * Dietary restrictions (vegetarian, gluten avoidance...) * Berries consumption \>1 serving/day * Pregnancy, breastfeeding or pregnancy planned within 3-6 months

Locations (1)

Laval University

Québec, Canada

Outcomes

Primary Outcomes

Changes in plasma insulin concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma glucose concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma triglycerides concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma total cholesterol concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma HDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma LDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Secondary Outcomes

Changes in waist circumference of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in hip circumference of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in waist to hip circumference ratio of overweight/obese men and women taking frozen mangos daily

Waist and hip circumference will be combined to report waist to hip ratio

Time frame: 8 weeks

Data from ClinicalTrials.gov

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Height of overweight/obese men and women taking frozen mangos daily

Time frame: Baseline

Changes in weight of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in body mass index (BMI) of overweight/obese men and women taking frozen mangos daily

Weight and height will be combined to report BMI

Time frame: 8 weeks

Changes in systolic blood pressure of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in diastolic blood pressure of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in insulin secretion of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in insulin sensitivity of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in glycated hemoglobin (HbA1c) in overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma tumor necrosis factor (TNF-alpha) concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma interleukin-6 (IL-6) concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma high-sensitivity C-reactive protein (hs-CRP) concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in gene expression in peripheral blood mononuclear cells (PBMC) of overweight/obese men and women taking frozen mangos daily

Gene-level expression from \>20,000 annotated genes will be examined

Time frame: 8 weeks

Changes in plasma metabolites concentration in overweight/obese men and women taking frozen mangos daily

Metabolites include acylcarnitines, glycerophospholipids, sphingolipids, sugars, amino acids, biogenic amines

Time frame: 8 weeks

Changes in gut microbiota composition in overweight/obese men and women taking frozen mangos daily

Stool samples will be collected and whole metagenome shotgun sequencing analysis will be conducted

Time frame: 8 weeks

Changes in plasma lipopolysaccharide (LPS) concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks

Changes in plasma LPS binding protein (LBP) concentration of overweight/obese men and women taking frozen mangos daily

Time frame: 8 weeks