Individuals with pre-diabetes or diabetes would benefit from low impact methods that would improve their insulin sensitivity and aid in maintaining glucose homeostasis. Physical activity helps the body decrease its insulin resistance and burn excess sugar. Many diabetics also suffer from obesity and specific forms or durations of physical exercise may not be viable options for these individuals. Determining whether short bursts of moderate exercise improve blood glucose levels in healthy humans may identify a further method for diabetics to improve their glucose homeostasis. The aim of this study is to determine the most effective moderate exercise that can be completed in 3 minutes and its effectiveness on improving glucose handling, in response to a single day of standardized high-fat and high-carbohydrate feeding in comparison to periods of prolonged sitting.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
6
3-hour sedentary period with 3 minute activity breaks of moderate exercise every 30 minutes
University of Prince Edward Island
Charlottetown, Prince Edward Island, Canada
Change in Insulin levels
The change in blood insulin levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).
Time frame: Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days
Change in concentration of C-peptide
The change in blood C-peptide levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).
Time frame: Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days
Changes in blood glucose levels
Blood glucose levels will be determined at pre-prandial baseline and over the following 3-hour testing period using hand-held blood glucose meters.
Time frame: Baseline and every 30-60 minutes over 3 hours (6 timepoints per test day)
Change in metabolomic response to a test meal
Targeted metabolomics analysis of approximately 139 metabolites in blood serum by LC-MS/MS to generate a metabolomic profile in response to a high-fat and high-carbohydrate test meal. Metabolites to be determined are categorized as biogenic amines, amino acids, histidines, carboxylic acids, acylcarnitines, glycerophospholipids and organic acids.
Time frame: Pre-prandial and 3-hours post test meal through study completion
Immediate effects of short-term sedentary behaviour on cognitive function
Assessing cognitive abilities using the Stroop Test.
Time frame: 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function
Assessing cognitive abilities using the Iowa Trail Making Test.
Time frame: 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function
Assessing cognitive abilities using the Digit-Span test.
Time frame: 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function
Assessing cognitive abilities using the N-back test.
Time frame: 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Immediate effects of short-term sedentary behaviour on cognitive function
Assessing cognitive abilities using the Flankers test.
Time frame: 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion
Gene expression levels in isolated PBMC populations
Determining changes in gene expression in pathways associated with RAGE signalling.
Time frame: Pre-prandial and 3 hours post-prandial on on test days through study completion
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