The aim of this study is to compare prepuce tissue samples from healthy male children who applied for circumcision and children with isolated hypospadias. Estrogen receptor 1 (ER1), androgen receptor (AR), FGFR2, transforming growth factor beta (TGF beta) and HOXA13 gene expression in the prepuce is planned to investigate via polymerase chain reaction (PCR) method. Mucosal vascular and nerve densities, and extracellular matrix density of laminin and collagen protein is planned to investigate via immunohistochemical examination.
Study Type
OBSERVATIONAL
Enrollment
52
Trakya University Faculty of Medicine
Edirne, Turkey (Türkiye)
Genetic analysis 1
Androgen receptor (AR) gene PCR analysis
Time frame: 1 year
Genetic analysis 2
Estrogen receptor 1 (ER1) gene PCR analysis
Time frame: 1 year
Genetic analysis 3
FGFR2 gene PCR analysis
Time frame: 1 year
Genetic analysis 4
TGF beta gene PCR analysis
Time frame: 1 year
Genetic analysis 5
HOXA13 gene PCR analysis
Time frame: 1 year
Immunohistochemical study 1
Mucosal vessel density is planned to determine via CD34
Time frame: 1 year
Immunohistochemical study 2
Mucosal nerve density is planned to determine via PGP 9.5
Time frame: 1 year
Histological study 1
Laminin is planned to determine in extracellular matrix via light microscope
Time frame: 1 year
Histological study 2
Collagen is planned to determine in extracellular matrix via light microscope
Time frame: 1 year
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.