Due to the long half-life (\~36 hr) of clenbuterol, detection methods such as dried blood spots (DBS) are a potentially suitable method to easily and non-invasively detect doping misuse of this compound for several days after ingestion. If, and how long, the compound can be detected by DBS has not yet been investigated but is of interest due to its potential in doping-control. The aim is to evaluate whether abuse of clenbuterol can be detected at relevant concentration levels in samples obtained using DBS and to assess the physiological response to clenbuterol in skeletal muscle..
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
16
Subjects ingest 4x20 microgram clenbuterol tablets
Subjects ingest placebo tablets
August Krogh Building
Copenhagen, Denmark
Blood clenbuterol concentration
Concentration of clenbuterol in dried blood spots
Time frame: Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of clenbuterol
Blood clenbuterol concentration
Concentration of clenbuterol in venous blood
Time frame: Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of study drug
Urine clenbuterol concentration
Concentration of clenbuterol in urine
Time frame: Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of study drug
Muscle strength
Maximal voluntary isometric contraction in N/m2 of the quadriceps
Time frame: Before (baseline) and 2.5 hours after administration of study drug
Muscle signalling
Protein kinase A phosphorylation in vastus lateralis biopsies
Time frame: Before (baseline) and 2.5 hours after administration of study drug
Plasma K+
Venous plasma K+ concentration
Time frame: Before (baseline) as well as 2.5 hours after administration of study drug
Muscle mTOR signalling
mTOR phosphorylation in vastus lateralis biopsies
Time frame: Before (baseline) as well as 2.5 hours after administration of study drug
Metabolic rate
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Respriatory exchange ratio of oxygen and CO2
Time frame: Before (baseline) as well as 2.5 hours after administration of study drug
Maximal voluntary strength
Maximal voluntary isometric muscle strength of the quadriceps
Time frame: Before (baseline) and after two-week treatment of study drug
Body composition
Lean and fat mass during a DXA-scan
Time frame: Before (baseline) and after two-week treatment of study drug
Aerobic capacity
Maximal oxygen uptake during maximal exercise
Time frame: Before (baseline) and after two-week treatment of study drug