Forearm Immobilization, Metabolic Health, and Muscle Loss

University of Exeter37 enrolled

Overview

The present study will investigate the impact of altered substrate availability on muscle atrophy, insulin sensitivity and muscle protein synthesis following short-term forearm immobilization

Thirty-six healthy young volunteers will undergo 2 days of forearm immobilization combined with ingestion of one of two drug or placebo. Before and after immobilization, they will receive a stable isotope tracer infusion (5.5 h) combined with repeated blood and muscle sampling under insulin clamp conditions, in order to measure insulin sensitivity and muscle protein synthesis in the fasted and fed state.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

DOUBLE

Enrollment

Conditions

Healthy

Interventions

Forearm immobilizationBEHAVIORAL

Two days of forearm immobilization

Eligibility

Sex: ALLMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Males and females 18-40 years of age * Body mass index between 18 and 27 Exclusion Criteria: * Any diagnosed metabolic impairment (e.g. type 1 or 2 diabetes) * Any diagnosed cardiovascular disease * Hypertension (≥140 mmHg systolic and/or ≥90 mmHg diastolic) * Chronic use of any prescribed or over the counter pharmaceuticals (excluding oral contraceptives and contraceptive devices) * Regular use of nutritional supplements * Metallic implants * A personal or family history of thrombosis, epilepsy, seizures or schizophrenia * Any previous motor disorders * Any known disorders in lipid metabolism * Any known disorders in muscle metabolism * Known allergy for Acipimox, beta agonist, or other substances in the tablets * Known sensitivity for sympathomimetic drugs * Known hypokalaemia * Presence of an ulcer in the stomach or gut and/or strong history of indigestion * Known severe kidney problems * Pregnancy * Unable to give consent

Locations (1)

Royal Exeter & Devon NHS Foundation Trust

Exeter, Devon, United Kingdom

Outcomes

Primary Outcomes

Percent Change in Forearm Glucose Uptake

Insulin sensitivity, measured as forearm glucose uptake, during a 30-min baseline period and hyperinsulinaemic-euglycaemic conditions

Time frame: During the steady-state phase of the insulin clamp (i.e. last 30 min)

Secondary Outcomes

Percent Change in Muscle Protein Synthesis

Percent change in muscle protein synthesis, measured as using the arteriovenous-venous method, via stable isotope tracer infusion

Time frame: In the fasted state (30 min before starting insulin clamp), and during the steady-state phase of the insulin clamp (i.e. last 30 min)

Data from ClinicalTrials.gov

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