It is not known how much improvement in insulin regulated lipolysis (the breakdown of triglycerides) occurs following substantial, sustained weight loss. Researchers will test the effects of inflammation and lipolysis regulation in people before and after bariatric surgery (sleeve gastrectomy) to answer these questions.
The purpose of this study is to determine whether the adipose inflammatory cell and cytokine content in Class III obesity is related to lipolysis insulin resistance and, if so, whether sustained, substantial weight loss one year following bariatric surgery reduces inflammation in parallel with improved insulin regulation of lipolysis
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
30
weight loss
Mayo Clinic
Rochester, Minnesota, United States
RECRUITINGadipose macrophage content, adipose IL-6 and TNF gene expression
Adipose tissue macrophage content as assessed by immunohistochemistry using stains for CD36, CD14 and CD206 will be done. mRNA expression for IL-6 and TNF will be measured
Time frame: Before bariatric surgery and approximately 1 year after surgery
insulin regulation of lipolysis (IC50)
The ability of insulin to suppress FFA palmitate release will be assessed using a 2-step insulin clamp and isotope dilution techniques for palmitate kinetics
Time frame: Before bariatric surgery and approximately 1 year after surgery
adipocyte perilipin 1 and HSL and ATGL
These proteins will be measured using capillary Western methods.
Time frame: Before bariatric surgery and approximately 1 year after surgery
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