Dose Escalating Study of Intramuscular Invaplex[AR-DETOX]

Inclusion Criteria: * Healthy, adult, male or female, age 18 to 50 years (inclusive) at the time of enrollment. * Completion and review of comprehension test (achieved ≥ 70% accuracy, two attempts allowed). * Provide written informed consent before initiation of any study procedures. * Agrees to complete all study visits and procedures and to provide a screening stool sample. * Women of childbearing capacity: Negative pregnancy test with understanding (through informed consent process) to not become pregnant during the study or within three (3) months following the last vaccine dose. Exclusion Criteria: * Health problems (for example, chronic medical conditions such as psychiatric conditions, diabetes mellitus, hypertension, or any other conditions that might place the subjects at increased risk of adverse events) - study clinicians, in consultation with the Principal Investigator (PI), will use clinical judgment on a case-by-case basis to assess safety risks under this criterion. The PI will consult with the Research Monitor as appropriate. * History of autoimmune disorders, cardiovascular and renal disease. * Use of immunosuppressive medications (systemic corticosteroids or chemotherapeutics that may influence antibody development), or immunosuppressive illness, including immunoglobulin A (IgA) deficiency (defined by serum IgA \< 7 mg/dL). * Women who are pregnant or planning to become pregnant during the study period plus 3 months beyond the last vaccine dose and currently nursing women. * Participation in research involving another investigational product (defined as receipt of an investigational product or exposure to an invasive investigational device) 30 days before planned date of first vaccination or anytime throughout the duration of the study until the last in-clinic study safety visit. * Positive blood test for hepatitis B surface antigen (HBsAG), hepatitis C virus antibody (HCV Ab), human immunodeficiency virus (HIV)-1/HIV-2 antibody. * Clinically significant abnormalities on basic laboratory screening tests. * Systemic antimicrobial treatment (i.e., topical treatments are not an exclusion) within 1 week before administration of the first vaccine dose. * Allergies that may increase the risk of adverse events (AEs). * Regular use (weekly or more often) of antidiarrheal, anti-constipation, or antacid therapy. * Abnormal stool pattern (fewer than 3 stools per week or more than 3 stools per day) on a regular basis; loose or liquid stools on other than an occasional basis. * Personal or family history of an inflammatory arthritis. * Positive blood test for human leukocyte antigen (HLA) B27 (associated with increased risk of reactive arthritis secondary to Shigella infection) * History of allergy to any vaccine. * Exclusionary skin disease history/findings that would confound assessment or prevent appropriate local monitoring of AEs, or possibly increase the risk of a local AE. * Serum immunoglobulin G (IgG) titer \> 2500 to Shigella flexneri 2a lipopolysaccharide antigen (LPS). * History of microbiologically confirmed Shigella infection. * Received previous licensed or experimental Shigella vaccine or live Shigella challenge. * Travel to countries where Shigella or other enteric infections are endemic (most of the developing world) within two years prior to dosing (clinician judgement). * Occupation involving handling of Shigella bacteria currently, or in the past 3 years.