Distribution of Nutrient Derived Amino Acids

Bispebjerg Hospital24 enrolled

Overview

The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

TRIPLE

Enrollment

Conditions

Muscle Mass Protein Intake Diet Modification Protein Synthesis Protein Breakdown Sarcopenia

Interventions

Distribution pattern of dietary proteins (minced meat)OTHER

Study effect of an even or skewed distribution pattern of dietary protein

Eligibility

Sex: ALLMin age: 65 YearsMax age: 80 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Men and women * Between 65-80 years of age Exclusion Criteria: * BMI above 30 * Smokers * Vegetarians * Diabetes or other metabolic diseases * Medical treatment which affects protein turnover * Gastro-intestinal diseases, reduced kidney or liver function, or chronic inflammation diseases * Elevated blood-pressure or signs of atherosclerosis * More than 5 hours of regular exercise each week

Locations (1)

Institute of Sports Medicine Copenhagen

Copenhagen, Denmark

Outcomes

Primary Outcomes

Muscle protein synthesis

Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured.

Time frame: 3 days

Net protein balance

Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated.

Time frame: 10 hours

Secondary Outcomes

D5-phenylalanine incorporation

D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine

Time frame: 3 days

Muscle protein synthesis

Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion.

Time frame: 10 hours

Protein synthesis

Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.

Time frame: 10 hours

Protein breakdown

Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique.

Time frame: 10 hours

Phenylalanine oxidation

Phenylalanine oxidation measured during acute study

Data from ClinicalTrials.gov

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