Dietary Oxalate and Immune Cell Function

N/AActive Not RecruitingNCT03877276

University of Alabama at Birmingham82 enrolled

Overview

The purpose of this study is to evaluate mitochondrial function in white blood cells and platelets from healthy individuals following dietary oxalate intake.

Inflammation and dietary oxalate have been shown to play an important role in the development of kidney stones. Oxalate is a small molecule found in plants and plant-derived food. Individuals that have high oxalate intake have an increased risk of developing kidney stones. The co-investigators have previously shown that healthy subjects that consume dietary oxalate have increased plasma and urine oxalate. White blood cells are essential for the immune response and rely on the mitochondria to carry out important cell functions. The Principal Investigator's current research shows that patients with calcium oxalate kidney stones have decreased mitochondrial function in their immune cells. Using the expertise of the investigators, this study will test whether oxalate has a direct effect on mitochondrial function in immune cells from healthy subjects. This information could help us understand the role of oxalate on the immune system during kidney stone pathogenesis.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

TREATMENT

Masking

NONE

Enrollment

Conditions

Kidney Stone

Interventions

BreakfastDIETARY_SUPPLEMENT

a prepared meal consisting of pastry and 2 cooked egg whites

Spinach smoothieDIETARY_SUPPLEMENT

a prepared blended spinach smoothie, consisting of banana, avocado, orange juice, and spinach

Kale smoothieDIETARY_SUPPLEMENT

prepared blended kale smoothie, consisting of banana, avocado, orange juice, and kale

Eligibility

Sex: ALLMin age: 18 YearsMax age: 70 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Mentally competent adults who are able to read and comprehend the consent form, written in English * Male or Female * Age 18 to 70 years * Non-tobacco users * BMI between 19 and 27 * Willingness to abstain from vigorous exercise during study period * Normal blood comprehensive metabolic panel Exclusion Criteria: * Mentally incompetent adults who are unable to read or comprehend the consent form, written in English * Pregnant females * Active medical problems * History of kidney stones * Any medical disorder that could influence absorption or excretion of oxalate * BMI less than 19 or greater than 27 * Tobacco users * Current use of medications or dietary supplements

Locations (1)

University of Alabama at Birmingham

Birmingham, Alabama, United States

Outcomes

Primary Outcomes

Change in monocyte cellular energetics after 5 hours

Cellular bioenergetics (oxygen consumption rate) will be measured in isolated monocytes from human blood using a Seahorse XF Analyzer machine. Monocyte cellular bioenergetics will be measured before and after consuming a spinach smoothie (pre- and post-loads; Time 0 hours and 5 hours) to assess a change in monocyte cellular energetics after 5 hours.

Time frame: 0 hours - 5 hours

Data from ClinicalTrials.gov

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