Two-day Bed Rest, Insulin Sensitivity and Muscle Protein Synthesis

University of Exeter

Overview

The impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

To study the mechanisms underlying inactivity-induced muscle atrophy, the impact of two days bed rest versus two days of habitual activity on insulin sensitivity and cumulative muscle protein synthesis will be investigated in healthy young males

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Conditions

Healthy

Interventions

Habitual activityBEHAVIORAL

Two days of habitual physical activity, under normal free-living conditions

Bed restBEHAVIORAL

Two days of bed rest, to induce whole-body physical inactivity

Eligibility

Sex: MALEMin age: 18 YearsMax age: 40 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Male * Aged 18-40 Exclusion Criteria: * Smoker * Any diagnosed metabolic impairment (e.g. type 1 or 2 Diabetes) * Any diagnosed cardiovascular disease (e.g. deep vein thrombosis or hypertension) * Chronic use of any prescribed or over the counter pharmaceuticals (that may modulate muscle protein metabolism). * A personal or family history of thrombosis, epilepsy, seizures or schizophrenia. * Any known disorders in muscle metabolism * Regular use of nutritional supplements (i.e. ingestion more than twice per week during the last month) * Metallic implants (including heart pacemaker, cochlear implants, medication pumps, surgical clips, plates or screws). * Allergy to lidocaine * Recent (within the last 6 months) or current musculoskeletal injury (e.g. leg fracture)

Outcomes

Primary Outcomes

Change in whole-body insulin sensitivity

Change in whole-body insulin sensitivity via meal tolerance test

Time frame: During 2 days of habitual physical activity and 2 days of bed rest. Blood glucose and insulin are monitored for 2 hours following ingestion of a standardized meal

Secondary Outcomes

Change in cumulative muscle protein synthesis

Cumulative muscle protein synthesis via deuterium oxide ingestion. The incorporation of deuterated alanine in muscle is expressed relative to body water enrichments, to obtain muscle protein synthesis in %/day

Time frame: During 2 days of habitual physical activity and 2 days of bed rest

Change in continuous glucose

Change in continuous glucose via continuous glucose monitoring.

Time frame: Continuous glucose, during the 2 days of habitual activity and 2 days of bed rest

Change in muscle volume

Change in muscle volume via MRI

Time frame: Immediately before and immediately after the 2-day bed rest period

Data from ClinicalTrials.gov

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